Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
National Security Education Center, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Biosensors (Basel). 2022 Mar 25;12(4):195. doi: 10.3390/bios12040195.
Rapid, on-site diagnostics allow for timely intervention and response for warfighter support, environmental monitoring, and global health needs. Portable optical biosensors are being widely pursued as a means of achieving fieldable biosensing due to the potential speed and accuracy of optical detection. We recently developed the portable engineered analytic sensor with automated sampling (PEGASUS) with the goal of developing a fieldable, generalizable biosensing platform. Here, we detail the development of PEGASUS's sensing hardware and use a test-bed system of identical sensing hardware and software to demonstrate detection of a fluorescent conjugate at 1 nM through biotin-streptavidin chemistry.
快速、现场诊断可实现及时干预和响应，以支持作战人员、进行环境监测和满足全球健康需求。由于光学检测具有潜在的速度和准确性，便携式光学生物传感器正被广泛研究，作为实现现场生物传感的手段。我们最近开发了具有自动采样功能的便携式工程分析传感器 (PEGASUS)，旨在开发一种可现场应用、可推广的生物传感平台。在这里，我们详细介绍了 PEGASUS 的传感硬件的开发，并使用相同的传感硬件和软件测试床系统演示了通过生物素-链霉亲和素化学检测 1 nM 的荧光缀合物。
