Oral Medicine, Department of Diagnosis and Surgery, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil.
Department of Orthodontics and Pediatric Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil.
Immunobiology. 2021 May;226(3):152072. doi: 10.1016/j.imbio.2021.152072. Epub 2021 Feb 23.
Macrophages are phagocytic cells with essential participation in immunological events of the oral cavity. However, the role of these cells in oral lichen planus (OLP) and oral lichenoid lesions (OLL) remains unclear. The present study aimed to evaluate the density of macrophages in OLP and OLL, and to compare it with that of oral inflammatory fibrous hyperplasia (OIFH) (control group). 14 cases of OLP, 14 cases of OLL and 14 cases of OIFH were selected for immunohistochemical analysis of CD68 (M1) and CD163 (M2) macrophage expression. CD68 and CD163 macrophages densities were measured in the intraepithelial and subepithelial areas. The statistical tests used were multivariate analysis of variance, as well as a correlation and linear regression. OLP has more CD68+ macrophages when comparing with OLL (p = 0.001) and OIFH (p = 0.045). There is a very strong relationship between the macrophages types (p < 0.0001) in OLP and OLL. The linear regression showed that to OLL development (p < 0.0001/R = 0.9584), the presence of different types of macrophages are more essential than to OLP (p < 0.0001/R = 0.8983). However, in the OLP these dependencies are also largely. CD68 macrophages may be associated with immunopathogenesis of OLP, indicating a pro-inflammatory activity and regulatory role in the type of T-cell response. Besides, CD68 macrophages can cooperate in the diagnosis of OLP. These results are essential to future studies that seek a therapeutic target for OLP and OLL.
巨噬细胞是具有吞噬作用的细胞，在口腔免疫事件中具有重要作用。然而，这些细胞在口腔扁平苔藓（OLP）和口腔类扁平苔藓病变（OLL）中的作用尚不清楚。本研究旨在评估 OLP 和 OLL 中巨噬细胞的密度，并将其与口腔炎症纤维性增生（OIFH）（对照组）进行比较。选择 14 例 OLP、14 例 OLL 和 14 例 OIFH 进行 CD68（M1）和 CD163（M2）巨噬细胞表达的免疫组织化学分析。测量上皮内和上皮下区域的 CD68 和 CD163 巨噬细胞密度。使用的统计检验方法是多变量方差分析，以及相关性和线性回归。与 OLL 和 OIFH 相比，OLP 中具有更多的 CD68+巨噬细胞（p=0.001 和 p=0.045）。OLP 和 OLL 中巨噬细胞类型之间存在很强的关系（p<0.0001）。线性回归显示，OLP 向 OLL 发展（p<0.0001/R=0.9584），不同类型的巨噬细胞的存在比 OLP 更为重要（p<0.0001/R=0.8983）。然而，在 OLP 中这些依赖性也很大。CD68 巨噬细胞可能与 OLP 的免疫发病机制有关，表明其在 T 细胞反应的炎症和调节作用。此外，CD68 巨噬细胞可辅助 OLP 的诊断。这些结果对未来研究寻找 OLP 和 OLL 的治疗靶点至关重要。
