Pak J Biol Sci. 2021 Jan;24(1):165-171. doi: 10.3923/pjbs.2021.165.171.
Andaliman fruit (Zanthoxylum acanthopodium) is a well-known spice antioxidant in Northern Sumatera (Indonesia). The cellular activity requires antioxidants in counteracting free radicals. The cellular proteins that play a role in development, proliferation, differentiation and embryonic processes in the human placenta are NOTCH1 and Hes1. The aim of this research was to analyze the expression of NOTCH1 and Hes1 genes after administering nano herbal andaliman to the trophoblast cells of the human placenta.
HTR8 trophoblast cells were divided into two groups, namely, control and treatment (nano herbal andaliman). RNA isolation, reverse transcription and RT-PCR (real-time polymerase chain reaction) were performed to analyze the NOTCH1 and Notch target gene (Hes1) expressions. The NOTCH1 and Hes1 gene expressions were quantified using the CT method (2-ΔΔCT) and normalized with Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expressions.
Nanoherbal andaliman reduced the expression of NOTCH1 genes in the human placental trophoblast. However, it increased the expression of Hes1 when the incubation time was 16 hrs.
Nanoherbal andaliman decreases the expression of genes that are crucial in hypoxia and free radicals in the placenta, namely, NOTCH1 and Hes1 increased after incubation for 16 hrs. Therefore, this herb needs to be evaluated further.
安汶果（Zanthoxylum acanthopodium）是北苏门答腊（印度尼西亚）著名的香料抗氧化剂。细胞活性需要抗氧化剂来对抗自由基。在人类胎盘的发育、增殖、分化和胚胎过程中起作用的细胞蛋白是 NOTCH1 和 Hes1。本研究旨在分析给予纳米草本安汶果后胎盘滋养细胞中 NOTCH1 和 Hes1 基因的表达。
HTR8 滋养细胞分为两组，即对照组和治疗组（纳米草本安汶果）。进行 RNA 分离、反转录和 RT-PCR（实时聚合酶链反应），以分析 NOTCH1 和 Notch 靶基因（Hes1）的表达。使用 CT 法（2-ΔΔCT）对 NOTCH1 和 Hes1 基因表达进行定量，并与甘油醛-3-磷酸脱氢酶（GAPDH）基因表达进行归一化。
纳米草本安汶果降低了人胎盘滋养细胞中 NOTCH1 基因的表达。然而，在孵育 16 小时后，它增加了 Hes1 的表达。
纳米草本安汶果降低了胎盘缺氧和自由基中关键基因的表达，即 NOTCH1 和 Hes1 在孵育 16 小时后增加。因此，这种草药需要进一步评估。
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