Simanullang Rostime Hermayerni, Situmorang Putri Cahaya, Herlina Meriani, Silalahi Bernita, Manurung Sarida Surya
Sekolah Tinggi Ilmu Kesehatan Murni Teguh, Medan, Indonesia.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.
Saudi J Biol Sci. 2022 Apr;29(4):2706-2718. doi: 10.1016/j.sjbs.2021.12.065. Epub 2022 Jan 4.
Cervical cancer is the second most lethal cancer in Indonesia, behind breast cancer. One of the reasons cancer cells are difficult to treat is that the immune system is sometimes unable to recognise them as foreign. Cytokinin therapy is carried out so that the immune system can strengthen its response to cancer cells, with the aim of slowing or stopping the development of malignant cells. DC, also known as andaliman, is an Indonesian herb and a member of the Rutaceae family. It is rich in antioxidants and has anti-inflammatory and anti-cancer properties. The current study aimed to investigate the histological changes and changes in the expression of cytokines, such as IL-10, IL1β, VEGFR1, and TGFβ1, associated with andaliman treatment. Sample tissues and serums extracted from cervical cancer rat models were used. Rats were divided into five groups: a control group (C-), cancer model group (C+), cancer with a dose of methanolic extract (ZAM) 100 mg/body weight (BW) ZAM (ZAM100), cancer with a dose of ZAM 200 mg/BW ZAM (ZAM200), and cancer with a dose of ZAM 400 mg/BW ZAM (ZAM400). Treatment lasted for 1 month. Blood samples were prepared for ELISA analysis, and cervical tissue was stained for immunohistochemistry using antibodies against IL-10, IL-1β, VEGFR1, and TGFβ1. Administration of ZAM had no significant effect on rat body weight and cervical organs (p > 0.05). However, it impacted haematological parameters in rats with cervical cancer (p < 0.05). Elevated malondialdehyde levels may be linked to superoxide dismutase deficiency in tumour tissue. ZAM significantly decreased the expression of IL1β, TGFβ1, and VEGFR1 (p < 0.01), while it increased the expression of IL-10. Therefore, ZAM may be a potential target for molecular cytokine therapy for cervical cancer.
宫颈癌是印度尼西亚第二大致命癌症，仅次于乳腺癌。癌细胞难以治疗的原因之一是免疫系统有时无法将它们识别为外来物质。进行细胞因子疗法以便免疫系统能够增强其对癌细胞的反应，目的是减缓或阻止恶性细胞的发展。DC，也被称为印尼山胡椒，是一种印尼草药，属于芸香科。它富含抗氧化剂，具有抗炎和抗癌特性。当前的研究旨在调查与印尼山胡椒治疗相关的组织学变化以及细胞因子如白细胞介素-10（IL-10）、白细胞介素-1β（IL1β）、血管内皮生长因子受体1（VEGFR1）和转化生长因子β1（TGFβ1）表达的变化。使用从宫颈癌大鼠模型中提取的样本组织和血清。大鼠被分为五组：对照组（C-）、癌症模型组（C+）、给予剂量为100毫克/体重（BW）的甲醇提取物（ZAM）的癌症组（ZAM100）、给予剂量为200毫克/BW ZAM的癌症组（ZAM）200）以及给予剂量为400毫克/BW ZAM的癌症组（ZAM400）。治疗持续1个月。制备血样用于酶联免疫吸附测定（ELISA）分析，并且使用针对IL-10、IL-1β、VEGFR1和TGFβ1的抗体对宫颈组织进行免疫组织化学染色。给予ZAM对大鼠体重和宫颈器官没有显著影响（p＞0.05）。然而，它对患有宫颈癌的大鼠的血液学参数有影响（p＜0.05）。丙二醛水平升高可能与肿瘤组织中超氧化物歧化酶缺乏有关。ZAM显著降低了IL1β、TGFβ1和VEGFR1的表达（p＜0.01），而它增加了IL-10的表达。因此，ZAM可能是宫颈癌分子细胞因子疗法的一个潜在靶点。
