Teles Amanda Mara, Silva-Silva João Victor, Fernandes Juan Matheus Pereira, Abreu-Silva Ana Lucia, Calabrese Kátia da Silva, Mendes Filho Nestor Everton, Mouchrek Adenilde Nascimento, Almeida-Souza Fernando
Pós-Graduação em Saúde do Adulto, Universidade Federal do Maranhão, São Luís 65065-545, Brazil.
Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil.
Evid Based Complement Alternat Med. 2021 Feb 20;2021:6663255. doi: 10.1155/2021/6663255. eCollection 2021.
has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil of . Eugenol (53.23%) as the major compound was verified by gas chromatography-mass spectrometry. essential oil was more effective against (MIC 50 g/mL) than eugenol (MIC 250 g/mL). Eugenol presented higher antioxidant activity than essential oil, with an EC of 12.66 and 78.98 g/mL, respectively. essential oil and eugenol exhibited inhibitory activity, with IC of 28.68 ± 1.073 and 31.97 ± 1.061 g/mL against epimastigotes and IC of 64.51 ± 1.658 and 45.73 ± 1.252 g/mL against intracellular amastigotes, respectively. Both compounds presented low cytotoxicity, with essential oil displaying 15.5-fold greater selectivity for the parasite than the cells. Nitrite levels in -stimulated cells were reduced by essential oil (47.01%; = 0.002) and eugenol (48.05%; = 0.003) treatment. The trypanocidal activity of essential oil showed that it is reasonable to use it in future research in the search for new therapeutic alternatives for trypanosomiasis.
由于其植物产品中发现的化合物，如总酚类化合物和黄酮类化合物，具有多种生物活性。本研究描述了[植物名称]精油的化学分析以及抗菌、抗氧化和抗锥虫活性。通过气相色谱 - 质谱法验证了丁香酚（53.23%）为主要化合物。[植物名称]精油对[病原体名称]（MIC 50μg/mL）比丁香酚（MIC 250μg/mL）更有效。丁香酚的抗氧化活性高于[植物名称]精油，其EC50分别为12.66和78.98μg/mL。[植物名称]精油和丁香酚均表现出抗锥虫活性，对前鞭毛体的IC50分别为28.68±1.073和31.97±1.061μg/mL，对细胞内无鞭毛体的IC50分别为64.51±1.658和45.73±1.252μg/mL。两种化合物的细胞毒性都很低，[植物名称]精油对寄生虫的选择性比对细胞高15.5倍。用精油（47.01%；P = 0.002）和丁香酚（48.05%；P = 0.003）处理可降低[刺激细胞的物质名称]刺激的细胞中的亚硝酸盐水平。[植物名称]精油的杀锥虫活性表明，在未来寻找锥虫病新治疗方法的研究中使用它是合理的。
