Laboratory of Biomaterial Surfaces & Interfaces, Institute of New Carbon Materials, Taiyuan University of Technology, Taiyuan 030024, China.
Second Hospital of Shanxi Medical University, Taiyuan 030024, China.
ACS Biomater Sci Eng. 2021 Apr 12;7(4):1438-1449. doi: 10.1021/acsbiomaterials.0c01588. Epub 2021 Mar 10.
Microbial infection and the limitation of tissue regeneration are main obstacles to chronic wound healing. Herein, a biofunctional hydrogel is prepared to simultaneously kill bacteria efficiently and promote would healing. First, a rose bengal/polypyrrole hybrid poly(vinyl alcohol) hydrogel (RB/PPy PVA HD) is synthesized and its antibacterial property is investigated under coirradiation of 550 nm visible light and 808 near-infrared light. The hydrogel exhibits excellent antibacterial activity within 10 min below 45 °C in vitro due to the synergistic effect of photothermal and photodynamic antibacterial therapy. Next, the recombined human epidermal growth factor (rhEGF) is physically absorbed on the surface of the porous hydrogel to form a RB/PPy/rhEGF hybrid PVA HD (rhEGF/RB/PPy PVA HD). The introduction of rhEGF enables the hydrogel to promote fibroblast proliferation and collagen secretion. Furthermore, the in vivo results indicate that the rhEGF/RB/PPy PVA HD can control infection effectively and promote wound healing significantly.
微生物感染和组织再生能力有限是慢性伤口愈合的主要障碍。在此，我们制备了一种具有生物功能的水凝胶，以同时高效杀灭细菌并促进伤口愈合。首先，合成了玫瑰红/聚吡咯杂化聚（乙烯醇）水凝胶（RB/PPy PVA HD），并研究了其在 550nm 可见光和 808nm 近红外光协同辐照下的抗菌性能。该水凝胶在 45°C 以下体外 10 分钟内即可表现出优异的抗菌活性，这是由于光热和光动力抗菌治疗的协同作用。接下来，将重组人表皮生长因子（rhEGF）物理吸附在多孔水凝胶的表面，形成 RB/PPy/rhEGF 杂化 PVA HD（rhEGF/RB/PPy PVA HD）。rhEGF 的引入使水凝胶能够促进成纤维细胞增殖和胶原分泌。此外，体内结果表明，rhEGF/RB/PPy PVA HD 可以有效控制感染并显著促进伤口愈合。
