从中光层海绵相关真菌 sp. NBUF082 中靶向分离细胞毒性环六肽
Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus sp. NBUF082.
机构信息
Department of Marine Pharmacy, Li Dak Sum Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China.
School of Marine Science, Ningbo University, Ningbo 315832, China.
出版信息
Mar Drugs. 2021 Oct 11;19(10):565. doi: 10.3390/md19100565.
LC-MS/MS-based molecular networking facilitated the targeted isolation of a new cyclic hexadepsipeptide, cymodepsipeptide (), and two known analogues, RF-2691A () and RF-2691B (), from the fungus sp. NBUF082 that was derived from a mesophotic zone sponge collected near Apo Island. The constitution and configuration of was elucidated through 1D and 2D NMR-spectroscopy, high resolution mass-spectrometry, and chemical degradations including Marfey's analysis and chiral HPLC. It was observed that was moderately cytotoxic against CCRF-CEM human acute lymphocytic leukemia cells in vitro with the IC value of 9.2 ± 1.1 μM.
基于 LC-MS/MS 的分子网络有助于靶向分离一种新型环状十六元环二肽，cymodepsipeptide ()，以及两种已知类似物，RF-2691A () 和 RF-2691B ()，它们来自于从中生带海绵中分离出来的真菌 sp. NBUF082，该海绵是在阿波岛附近采集的。通过 1D 和 2D NMR 光谱、高分辨率质谱和包括 Marfey 分析和手性 HPLC 的化学降解等方法，阐明了 的结构和构型。观察到 在体外对 CCRF-CEM 人急性淋巴细胞白血病细胞具有中等细胞毒性，IC 值为 9.2 ± 1.1 μM。
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