Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
Department of Pathology, Mongi Slim University Hospital, La Marsa, Tunisia.
Front Immunol. 2021 Feb 22;11:630139. doi: 10.3389/fimmu.2020.630139. eCollection 2020.
Extrahepatic cancers of the biliary system are typically asymptomatic until after metastasis, which contributes to their poor prognosis. Here we examined intrahepatic cholangiocarcinomas (n = 8), carcinomas of perihilar bile ducts (n = 7), carcinomas of the gallbladder (n = 11) and hepatic metastasis from carcinomas of the gallbladder (n = 4) for the expression of the extracellular matrix glycoproteins tenascin-C and tenascin-W. Anti-tenascin-C and anti-tenascin-W immunoreactivity was found in all biliary tract tumors examined. Unlike tenascin-C, tenascin-W was not detected in normal hepatobiliary tissue. Tenascin-W was also expressed by the cholangiocarcinoma-derived cell line Huh-28. However, co-culture of Huh-28 cells with immortalized bone marrow-derived stromal cells was necessary for the formation and organization of tenascin-W fibrils . Our results indicate that tenascin-W may be a novel marker of hepatobiliary tumor stroma, and its absence from many normal tissues suggests that it may be a potential target for biotherapies.
肝外胆道系统的癌症通常在转移后才出现症状，这导致了其预后不良。在这里，我们检查了 8 例肝内胆管癌、7 例肝门部胆管癌、11 例胆囊癌和 4 例胆囊癌肝转移，以研究细胞外基质糖蛋白 tenascin-C 和 tenascin-W 的表达情况。我们发现所有胆道肿瘤都有抗 tenascin-C 和抗 tenascin-W 免疫反应。与 tenascin-C 不同，tenascin-W 在正常肝胆组织中未检测到。tenascin-W 也在胆管癌衍生细胞系 Huh-28 中表达。然而，Huh-28 细胞与永生化骨髓基质细胞共培养对于 tenascin-W 纤维的形成和组织是必需的。我们的结果表明，tenascin-W 可能是肝胆肿瘤基质的一个新标志物，其在许多正常组织中缺失提示它可能是生物治疗的潜在靶点。
