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大豆中C2结构域家族的全基因组分析及一个假定的非生物胁迫响应基因的鉴定

Genome-Wide Analysis of the C2 Domain Family in Soybean and Identification of a Putative Abiotic Stress Response Gene .

作者信息

Sun Yue, Zhao Juan-Ying, Li Yi-Tong, Zhang Pei-Gen, Wang Shu-Ping, Guo Jun, Chen Jun, Zhou Yong-Bin, Chen Ming, Ma You-Zhi, Fang Zheng-Wu, Xu Zhao-Shi

机构信息

College of Agriculture, Yangtze University, Hubei Collaborative Innovation Center for Grain Industry, Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou, China.

Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, China.

出版信息

Front Plant Sci. 2021 Feb 16;12:620544. doi: 10.3389/fpls.2021.620544. eCollection 2021.

DOI:10.3389/fpls.2021.620544
PMID:33692816
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7939022/
Abstract

Plant C2 domain proteins play essential biological functions in numerous plants. In this study, 180 soybean C2 domain genes were identified by screening. Phylogenetic relationship analysis revealed that C2 domain genes fell into three distinct groups with diverged gene structure and conserved functional domain. Chromosomal location analysis indicated that C2 domain genes mapped to 20 chromosomes. The transcript profiles based on RNA-seq data showed that , , and had higher levels of expression under salt, drought, and abscisic acid (ABA) treatments. , encoding a cell membrane-localized protein, had the highest level of response to various treatments according to real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Under salt and drought stresses, the soybean plants with transgenic hairy roots showed delayed leaf rolling, a higher content of proline (Pro), and lower contents of HO, O and malondialdehyde (MDA) compared to those of the empty vector (EV) plants. The results of transgenic in salt and drought treatments were consistent with those in soybean treatments. In addition, the soybean plants with transgenic hairy roots increased transcript levels of several abiotic stress-related marker genes, including , , , , , , and which resulted in enhanced abiotic stress tolerance in soybean. These results indicate that C2 domain genes are involved in response to salt and drought stresses, and this study provides a genome-wide analysis of the C2 domain family in soybean.

摘要

植物C2结构域蛋白在众多植物中发挥着重要的生物学功能。在本研究中，通过筛选鉴定出180个大豆C2结构域基因。系统发育关系分析表明，C2结构域基因分为三个不同的组，具有不同的基因结构和保守的功能结构域。染色体定位分析表明，C2结构域基因分布在20条染色体上。基于RNA测序数据的转录谱显示，[此处原文缺失具体基因名称]在盐、干旱和脱落酸（ABA）处理下具有较高的表达水平。根据实时定量聚合酶链反应（RT-qPCR）分析，编码细胞膜定位蛋白的[此处原文缺失具体基因名称]对各种处理的反应水平最高。在盐和干旱胁迫下，与空载体（EV）植株相比，具有[此处原文缺失具体基因名称]转基因毛状根的大豆植株叶片卷曲延迟，脯氨酸（Pro）含量较高，过氧化氢（HO）、超氧阴离子（O）和丙二醛（MDA）含量较低。[此处原文缺失具体基因名称]转基因在盐和干旱处理中的结果与大豆处理中的结果一致。此外，具有[此处原文缺失具体基因名称]转基因毛状根的大豆植株提高了几个非生物胁迫相关标记基因的转录水平，包括[此处原文缺失具体基因名称]，这导致大豆对非生物胁迫的耐受性增强。这些结果表明，C2结构域基因参与了对盐和干旱胁迫的响应，本研究提供了大豆C2结构域家族的全基因组分析。

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4
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