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锚蛋白重复基因赋予水稻和大豆耐旱及耐盐性。

The Ankyrin-Repeat Gene Confers Drought and Salt Tolerance in and Soybean.

作者信息

Zhao Juan-Ying, Lu Zhi-Wei, Sun Yue, Fang Zheng-Wu, Chen Jun, Zhou Yong-Bin, Chen Ming, Ma You-Zhi, Xu Zhao-Shi, Min Dong-Hong

机构信息

College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, China.

Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, China.

出版信息

Front Plant Sci. 2020 Oct 29;11:584167. doi: 10.3389/fpls.2020.584167. eCollection 2020.

DOI:10.3389/fpls.2020.584167
PMID:33193533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7658197/
Abstract

Ankyrin repeat (ANK) proteins are essential in cell growth, development, and response to hormones and environmental stresses. In the present study, 226 genes were identified and classified into nine subfamilies according to conserved domains in the soybean genome ( L.). Among them, the was highly induced by drought, salt, and abscisic acid. The encodes a protein that belongs to the ANK-RF subfamily containing a RING finger (RF) domain in addition to the ankyrin repeats. Heterologous overexpression of in transgenic improved the germination rate under drought and salt treatments compared to wild-type. Homologous overexpression of improved the survival rate under drought and salt stresses in transgenic soybean hairy roots. In response to drought or salt stress, overexpression in soybean hairy root showed higher proline and lower malondialdehyde contents, and lower HO and O contents compared control plants. Besides, activated transcription of several abiotic stress-related genes, including , , , , and under drought and salt stresses in soybean. These results provide new insights for functional analysis of soybean ANK proteins and will be helpful for further understanding how ANK proteins in plants adapt to abiotic stress.

摘要

锚蛋白重复序列（ANK）蛋白在细胞生长、发育以及对激素和环境胁迫的响应中至关重要。在本研究中，根据大豆基因组（L.）中的保守结构域鉴定出226个基因，并将其分为九个亚家族。其中，该基因受干旱、盐和脱落酸的强烈诱导。该基因编码一种蛋白质，除了锚蛋白重复序列外，还属于含有一个环状结构域（RF）的ANK-RF亚家族。与野生型相比，该基因在转基因植物中的异源过表达提高了干旱和盐处理下的发芽率。该基因在转基因大豆毛状根中的同源过表达提高了干旱和盐胁迫下的存活率。响应干旱或盐胁迫，大豆毛状根中该基因的过表达与对照植株相比，脯氨酸含量更高，丙二醛含量更低，过氧化氢和超氧阴离子含量更低。此外，该基因在干旱和盐胁迫下激活了大豆中几个与非生物胁迫相关基因的转录，包括、、、和。这些结果为大豆ANK蛋白的功能分析提供了新的见解，并将有助于进一步了解植物中的ANK蛋白如何适应非生物胁迫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/b88abfa76f21/fpls-11-584167-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/4916192375e8/fpls-11-584167-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/ac8a42fe4ef8/fpls-11-584167-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/f3bb743425a6/fpls-11-584167-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/be0875f059cb/fpls-11-584167-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/4381023cfb3a/fpls-11-584167-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/569b5bc78188/fpls-11-584167-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/7866d789eca7/fpls-11-584167-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/b88abfa76f21/fpls-11-584167-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/4916192375e8/fpls-11-584167-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/2f8f48d0e880/fpls-11-584167-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/332c584fbb81/fpls-11-584167-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/628ad4114f55/fpls-11-584167-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/2f7f2f2fc15b/fpls-11-584167-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/ac8a42fe4ef8/fpls-11-584167-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/f3bb743425a6/fpls-11-584167-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/be0875f059cb/fpls-11-584167-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/4381023cfb3a/fpls-11-584167-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/569b5bc78188/fpls-11-584167-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/7866d789eca7/fpls-11-584167-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a66f/7658197/b88abfa76f21/fpls-11-584167-g012.jpg

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