Liu Chong, Chen Mingshi, Shi Yue
Department of Breast Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China.
Traditional Chinese Medicine Department, The First Affiliated Hospital of China Medical University, Shenyang, China.
Gland Surg. 2021 Feb;10(2):816-825. doi: 10.21037/gs-21-42.
Circular ribonucleic acids (circRNAs) are highly stable and conserved forms of RNAs present in all eukaryotes. They can modulate the expression of genes by sponging specific micro RNAs (miRNAs), thereby affecting various disease processes. However, their expression pattern in human breast cancer has not been elucidated.
In this study, differentially expressed circRNAs in breast cancer tissues and paired noncancerous tissues were analyzed using an Arraystar Human circRNA Microarray, and hsa_circ_0006220 was selected for its 27-fold downregulation in breast cancer tissues. Its expression was also verified in 50 breast cancer and paired noncancerous tissues using real-time polymerase chain reaction (RT-PCR). An analysis of the expression of hsa_circ_0006220 and the clinicopathological factors in breast cancer was conducted. A receiver operating characteristic (ROC) curve of hsa_circ_0006220 was constructed. The interaction between hsa_circ_0006220 and five possible target miRNAs was predicted, and their expression were verified when overexpressing hsa_circ_0006220 by RT-PCR.
Hsa_circ_0006220 was found to be significantly downregulated in breast cancer tissues compared to the paired noncancerous tissues by microarray and RT-PCR. The expression of hsa_circ_0006220 was significantly inversely correlated with histological type (P=0.0028) and lymph node metastasis (P=0.0341). The area under the ROC curve (AUC) was 0.706. Five miRNAs that might be sponged by hsa_circ_0006220 were predicted. MiR-197-5p was significantly downregulated after overexpression of hsa_circ_0006220.
Our results indicated that hsa_circ_0006220 may play a role in human breast cancer and might be a potential tumor marker for breast cancer screening.
环状核糖核酸（circRNAs）是存在于所有真核生物中的高度稳定且保守的RNA形式。它们可以通过吸附特定的微小RNA（miRNAs）来调节基因表达，从而影响各种疾病进程。然而，它们在人类乳腺癌中的表达模式尚未阐明。
在本研究中，使用Arraystar人类circRNA微阵列分析乳腺癌组织和配对的癌旁组织中差异表达的circRNAs，并选择在乳腺癌组织中下调27倍的hsa_circ_0006220进行研究。还使用实时聚合酶链反应（RT-PCR）在50例乳腺癌及配对的癌旁组织中验证其表达。对hsa_circ_0006220的表达与乳腺癌临床病理因素进行分析。构建hsa_circ_0006220的受试者工作特征（ROC）曲线。预测hsa_circ_0006220与5种可能的靶miRNAs之间的相互作用，并通过RT-PCR在过表达hsa_circ_0006220时验证它们的表达。
通过微阵列和RT-PCR发现，与配对的癌旁组织相比，hsa_circ_0006220在乳腺癌组织中显著下调。hsa_circ_0006220的表达与组织学类型（P = 0.0028）和淋巴结转移（P = 0.0341）显著负相关。ROC曲线下面积（AUC）为0.706。预测了5种可能被hsa_circ_0006220吸附的miRNAs。过表达hsa_circ_0006220后，miR-197-5p显著下调。
我们的结果表明，hsa_circ_0006220可能在人类乳腺癌中发挥作用，可能是乳腺癌筛查的潜在肿瘤标志物。
