Plant and Microbial Physiology and Biochemistry Laboratory, Department of Botany, University of Gour Banga, P.O. - Mokdumpur, Malda, WB, 732 103, India.
Department of Microbiology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal, 700 019, India.
Braz J Microbiol. 2021 Jun;52(2):905-917. doi: 10.1007/s42770-021-00439-w. Epub 2021 Mar 14.
The main objective of the study is to characterize two new strains of Aspergillus fumigatus through morphometric, biochemical, molecular methods, and to evaluate their antimicrobial potentiality. The micro-morphotaxonomy, growth, and metabolic behavior of the strains, nHF-01 and PPR-01, were studied in different growth conditions and compared with standard strain. The molecular characterization was done by sequencing the ncrDNA ITS1-5.8S-ITS2 and D1-D2 domains of the nc 28S rDNA region and compared with a secondary structure-based phylogenetic tree. The secretory antimicrobials and pigments were characterized by TLC, UV-Vis, and FT-IR spectroscopy. Both the strains showed distinct growth patterns in different nutritional media and could assimilate a wide range of carbohydrates with distinctive biochemical properties. The molecular characterization revealed the strains, nHF-01 and PPR-01, as Aspergillus fumigatus (GenBank Accession No. MN190286 and MN190284, respectively). It was observed that the strain nHF-01 produces red to brownish pigments having mild antimicrobial activity while the strain PPR-01 does not represent such transformations. The extractable compounds had a significant antimicrobial potentiality against the human pathogenic bacteria. From this analysis, it can be concluded that the nHF-01 and PPR-01 strains are distinct from other A. fumigatus by their unique characters. Large-scale production and detailed molecular elucidation of the antimicrobial compounds may lead to the discovery of new antimicrobial compounds from these strains.
本研究的主要目的是通过形态计量学、生物化学和分子方法来描述两株新型烟曲霉（Aspergillus fumigatus）的特征，并评估它们的抗菌潜力。对菌株 nHF-01 和 PPR-01 的微观形态分类学、生长和代谢行为进行了研究，在不同的生长条件下进行了研究，并与标准菌株进行了比较。通过对 nc 28S rDNA 区的 ncrDNA ITS1-5.8S-ITS2 和 D1-D2 区域进行测序，并与基于二级结构的系统发育树进行比较，进行了分子特征描述。通过 TLC、UV-Vis 和 FT-IR 光谱对分泌性抗生素和色素进行了表征。两种菌株在不同的营养培养基中表现出明显不同的生长模式，能够同化广泛的碳水化合物，具有独特的生化特性。分子特征表明，菌株 nHF-01 和 PPR-01 均为烟曲霉（GenBank 登录号分别为 MN190286 和 MN190284）。观察到菌株 nHF-01 产生红色到棕褐色色素，具有温和的抗菌活性，而菌株 PPR-01 则没有这种转化。可提取化合物对人类致病菌具有显著的抗菌潜力。从这项分析可以得出结论，nHF-01 和 PPR-01 菌株通过其独特的特性与其他烟曲霉菌株不同。大规模生产和对抗菌化合物的详细分子阐明可能会导致从这些菌株中发现新的抗菌化合物。
