Yao Yao, Dong Ziqing, Liao Yunjun, Zhang Pan, Ma Jingjing, Gao Jianhua, Lu Feng
Guangzhou, Guangdong, People's Republic of China.
From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University.
Plast Reconstr Surg. 2017 Apr;139(4):867-879. doi: 10.1097/PRS.0000000000003214.
Adipose-derived stem cells and other stromal vascular fraction cells were used more often for stem cell therapy, even though limitations such as poor cell retention rate, complicated and expensive isolation processes, and the use of specific laboratory equipment need to be overcome.
Here, the authors developed a novel but simple method for generating an injectable mixture of stromal vascular fraction cells and native adipose extracellular matrix. It is a purely mechanical process in which lipoaspirate is processed into an extracellular matrix/stromal vascular fraction gel. The standard processing procedure was established using quantized tests. The therapeutic potential of the product for wound healing was then tested.
Extracellular matrix/stromal vascular fraction gel derived from lipoaspirate and processed using a standard Coleman technique, followed by 1 minute of mechanical processing by passage back and forth between two 10-ml syringes at a flow rate of 10 ml/second, showed the highest adipose-derived stem cell and endothelial cell density. The stromal vascular fraction cells within the product also showed potential for multipotent differentiation similar to that of normal fat samples. In addition, the product showed better therapeutic results than stromal vascular fraction cell suspension when used to treat a nude mouse model of wound healing.
Extracellular matrix/stromal vascular fraction gel is an autologous injectable derived from native extracellular matrix and is a functional cellular component generated using a simple mechanical process. As such, it may offer a novel mode of tissue repair suitable for clinical application in stem cell therapies.
脂肪来源干细胞和其他基质血管成分细胞在干细胞治疗中应用更为频繁，尽管存在诸如细胞留存率低、分离过程复杂且昂贵以及需要使用特定实验室设备等局限性，仍需克服。
在此，作者开发了一种新颖但简单的方法来生成可注射的基质血管成分细胞与天然脂肪细胞外基质的混合物。这是一个纯粹的机械过程，其中脂肪抽吸物被加工成细胞外基质/基质血管成分凝胶。通过量化测试建立了标准加工流程。随后测试了该产品在伤口愈合方面的治疗潜力。
源自脂肪抽吸物并采用标准科尔曼技术处理，然后以10毫升/秒的流速在两个10毫升注射器之间来回机械处理1分钟的细胞外基质/基质血管成分凝胶，显示出最高的脂肪来源干细胞和内皮细胞密度。产品中的基质血管成分细胞也显示出与正常脂肪样本相似的多能分化潜力。此外，在用于治疗伤口愈合的裸鼠模型时，该产品比基质血管成分细胞悬液显示出更好的治疗效果。
细胞外基质/基质血管成分凝胶是一种源自天然细胞外基质的自体可注射物，是通过简单机械过程生成的功能性细胞成分。因此，它可能提供一种适用于干细胞治疗临床应用的新型组织修复模式。
