Xu Qiang, Peng Xiaoya, Wang Yanhong, Lu Lu, Zhang Yongping, Qin Kun, Liu Jia, Peng Fang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
School of Science, Tibet University, Lhasa 850000, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004744. Epub 2021 Mar 16.
A Gram-stain-negative, rod-shaped, and aerobic bacterium, strain 16-35-5, was isolated from Collins Glacier front soil from the Fildes Peninsula, Antarctica. The bacterium grew optimally at 28 °C, pH 7.0 and in the presence of 0-4.0 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence phylogenetic analyses, it was concluded that 16-35-5 represented a member of the genus and had the highest sequence similarities with CFBP 3232 (96.48 %). The genome of 16-35-5 is 4.2 Mb long with a DNA G+C content of 66.3 mol%. Average nucleotide identity (ANI) value between the genomes of 16-35-5 and DSM 27981, was 85.29 %. Strain 16-35-5 had ubiquinone-8 (Q-8) as the respiratory ubiquinone. The polar lipids of 16-35-5 were consisted of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The main fatty acids were summed feature 3 (Cω7 and/or Cω6, 25.2 %), summed feature 8 (Cω7 and/or Cω6, 12.9 %), C (35.2 %), and C cyclo (19.0 %). On the basis of the evidence presented in this study, 16-35-5 should be classified as representing a novel species of the genus , for which the name sp. nov., is proposed, with the type strain 16-35-5 (=CCTCC AB 2019325=KCTC 72915).
从南极菲尔德斯半岛柯林斯冰川前沿土壤中分离出一株革兰氏阴性、杆状需氧细菌，菌株16 - 35 - 5。该细菌在28°C、pH 7.0以及0 - 4.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列系统发育分析结果，得出结论：16 - 35 - 5代表该属的一个成员，与CFBP 3232的序列相似性最高（96.48%）。16 - 35 - 5的基因组长度为4.2 Mb，DNA G + C含量为66.3 mol%。16 - 35 - 5与DSM 27981基因组之间的平均核苷酸同一性（ANI）值为85.29%。菌株16 - 35 - 5以泛醌 - 8（Q - 8）作为呼吸泛醌。16 - 35 - 5的极性脂质由磷脂酰乙醇胺、二磷脂酰甘油和磷脂酰甘油组成。主要脂肪酸为总和特征3（Cω7和/或Cω6，25.2%）、总和特征8（Cω7和/或Cω6，12.9%）、C（35.2%）和C环（19.0%）。基于本研究提供的证据，16 - 35 - 5应被归类为该属的一个新物种，为此提出新物种名称为sp. nov.，模式菌株为16 - 35 - 5（=CCTCC AB 2019325=KCTC 72915）。
