sp. nov., a flavobacterium isolated from Antarctic soil.

A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped, non-motile, yellow-pigmented bacteria, designated strain G5-32, belonging to the genus was isolated from soil collected in the Antarctic. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0-5% (w/v) NaCl (optimum, 1%), at pH 6.0-9.0 (optimum, pH 8.0) and at 4-28 °C (optimum, 20 °C). The predominant menaquinone was MK-6 (99.4%). The major fatty acids were anteiso-C (28.2%), iso-C (16.4%), summed feature 9 (comprising iso-C 9 and/or 10-methyl C; 10.6%) and iso-C (5.9%). A phylogenetic tree based on 16S rRNA gene sequences showed that strain G5-32 formed a lineage within the genus with the closest phylogenetic neighbours HMD1043, DSM 23145, DSM 17048 and NCTC 13525 (97.9, 97.8, 97.8 and 98.0 % 16S rRNA gene sequence similarity, respectively). The ANI values between strain G5-32 and DSM 17048, DSM 23145, NCTC 13525 and HMD1043 were 90.9, 82.6, 77.1 and 76.3%. Concurrently, digital DNA-DNA hybridization values of strain G5-32 assessed against DSM 17048, DSM 23145, NCTC 13525 and HMD1043 were 42.3, 25.9, 21.7 and 21.3%, respectively. Based on phenotypic, phylogenetic and genotypic data, a novel species, sp. nov., is proposed. The type strain is G5-32 (=CCTCC AA 2019083=KCTC 72766).