Ren Xingtao, Jiang Peiqiang, Liu Zuoyang, Liang Yuzi, Li Jing
College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, 266003, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004753. Epub 2021 Mar 16.
A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped, non-motile, yellow-pigmented bacteria, designated strain G5-32, belonging to the genus was isolated from soil collected in the Antarctic. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0-5% (w/v) NaCl (optimum, 1%), at pH 6.0-9.0 (optimum, pH 8.0) and at 4-28 °C (optimum, 20 °C). The predominant menaquinone was MK-6 (99.4%). The major fatty acids were anteiso-C (28.2%), iso-C (16.4%), summed feature 9 (comprising iso-C 9 and/or 10-methyl C; 10.6%) and iso-C (5.9%). A phylogenetic tree based on 16S rRNA gene sequences showed that strain G5-32 formed a lineage within the genus with the closest phylogenetic neighbours HMD1043, DSM 23145, DSM 17048 and NCTC 13525 (97.9, 97.8, 97.8 and 98.0 % 16S rRNA gene sequence similarity, respectively). The ANI values between strain G5-32 and DSM 17048, DSM 23145, NCTC 13525 and HMD1043 were 90.9, 82.6, 77.1 and 76.3%. Concurrently, digital DNA-DNA hybridization values of strain G5-32 assessed against DSM 17048, DSM 23145, NCTC 13525 and HMD1043 were 42.3, 25.9, 21.7 and 21.3%, respectively. Based on phenotypic, phylogenetic and genotypic data, a novel species, sp. nov., is proposed. The type strain is G5-32 (=CCTCC AA 2019083=KCTC 72766).
从南极采集的土壤中分离出一株革兰氏阴性、需氧、无芽孢形成、杆状、不运动、产黄色色素的细菌，命名为菌株G5-32，属于该属。采用多相分类方法对该菌株进行鉴定。该菌株在0-5%（w/v）NaCl（最适浓度为1%）、pH 6.0-9.0（最适pH为8.0）和4-28°C（最适温度为20°C）条件下生长。主要的甲基萘醌是MK-6（99.4%）。主要脂肪酸为anteiso-C（28.2%）、iso-C（16.4%）、总和特征9（包括iso-C 9和/或10-甲基C；10.6%）和iso-C（5.9%）。基于16S rRNA基因序列构建的系统发育树表明，菌株G5-32在该属内形成了一个分支，与最接近的系统发育邻居HMD1043、DSM 23145、DSM 17048和NCTC 13525的16S rRNA基因序列相似性分别为97.9%、97.8%、97.8%和98.0%。菌株G5-32与DSM 17048、DSM 23145、NCTC 13525和HMD1043之间的ANI值分别为90.9%、82.6%、77.1%和76.3%。同时，菌株G5-32与DSM 17048、DSM 23145、NCTC 13525和HMD1043的数字DNA-DNA杂交值分别为42.3%、25.9%、21.7%和21.3%。基于表型、系统发育和基因型数据，提出了一个新物种，新种。模式菌株为G5-32（=CCTCC AA 2019083=KCTC 72766）。
