de Souza Beatriz Martines, Silva Mayara Souza, Braga Aline Silva, Bueno Patrícia Sanches Kerges, da Silva Santos Paulo Sergio, Buzalaf Marília Afonso Rabelo, Magalhães Ana Carolina
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-191, Brazil.
Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-191, Brazil.
Sci Rep. 2021 Mar 16;11(1):6083. doi: 10.1038/s41598-021-85748-8.
This in vitro study evaluated the protective effect of titanium tetrafluoride (TiF) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF and SDF significantly reduced the biofilm viability compared to negative control. TiF significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF and SDF may be good alternatives to control the development of radiation-induced dentin caries.
这项体外研究评估了四氟化钛（TiF）清漆和氟化亚锡（SDF）溶液对辐射诱导的牙本质龋的保护作用。对牛牙根牙本质样本进行辐照（70 Gy）并按以下方式处理：（6小时）：4% TiF清漆；5.42% NaF清漆；30% SDF溶液；安慰剂清漆；或不处理（阴性对照）。在最初8小时内，用人牙生物膜（来自头颈癌患者）与麦克贝恩唾液混合产生微观生物膜。16小时后以及从第2天到第5天，每天更换麦克贝恩唾液（0.2%蔗糖）（37°C，5%二氧化碳）（生物重复三次）。通过横向显微放射摄影（TMR）对脱矿进行定量，同时通过活力、菌落形成单位（CFU）计数和乳酸产生测定分析生物膜。数据通过方差分析进行统计学分析（p < 0.05）。与安慰剂和阴性对照相比，TiF和SDF能够减少矿物质损失。与阴性对照相比，TiF和SDF显著降低了生物膜活力。TiF显著降低了总微生物的CFU计数，而只有SDF影响总链球菌和变形链球菌计数。与阴性对照相比，清漆诱导乳酸产生减少。TiF和SDF可能是控制辐射诱导牙本质龋发展的良好替代方法。
