Jaffe C L, Zalis M
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
J Infect Dis. 1988 Jun;157(6):1212-20. doi: 10.1093/infdis/157.6.1212.
Serodiagnosis of visceral leishmaniasis (VL) due to Leishmania donovani by using crude parasite antigen is complicated in many endemic areas because of cross-reactions with sera from humans with Chagas' disease. We used affinity-purified parasite proteins to develop a direct dot-blot assay for VL. Double-blind tests were carried out on sera from 40 patients with well-documented VL, from controls in endemic areas, and from patients with other diseases. Using gp70-2, 36 (90.0%) of 40 sera from patients with kala azar were correctly diagnosed; 1 (1.2%) of 86 sera from patients without kala azar was misdiagnosed. With dp72, 21 (100%) of 21 sera from patients with VL were correctly identified; 5 (7.0%) of 71 negative sera were misdiagnosed. None of the 18 sera from patients with Chagas' disease reacted positively against gp70-2. Our assay is rapid, simple, and specific and represents a new method for reliably diagnosing and monitoring VL.
在许多流行地区，由于杜氏利什曼原虫引起的内脏利什曼病（VL）的血清学诊断因与恰加斯病患者血清发生交叉反应而变得复杂。我们使用亲和纯化的寄生虫蛋白开发了一种用于VL的直接斑点印迹检测方法。对来自40例确诊VL患者、流行地区对照以及其他疾病患者的血清进行了双盲检测。使用gp70-2，40例黑热病患者血清中有36例（90.0%）被正确诊断；86例无黑热病患者血清中有1例（1.2%）被误诊。使用dp72，21例VL患者血清中有21例（100%）被正确识别；71例阴性血清中有5例（7.0%）被误诊。18例恰加斯病患者的血清中无一例对gp70-2呈阳性反应。我们的检测方法快速、简单且特异，是一种可靠诊断和监测VL的新方法。
