一种制备富血小板血浆的新经济方法。

A New Economic Method for Preparing Platelet-rich Plasma.

作者信息

Fukaya Mototsugu, Ito Asako

机构信息

Tsurumai Kouen Clinic, Nagoya, Japan.

出版信息

Plast Reconstr Surg Glob Open. 2014 Jul 9;2(6):e162. doi: 10.1097/GOX.0000000000000109. eCollection 2014 Jun.

BACKGROUND

Although platelet-rich plasma (PRP) is nowadays a common method in various medical fields, including cosmetic surgery or dermatology, the expensiveness of the kit for processing is still a hurdle.

METHODS

A new unique economic method for preparing PRP was reported. The method consists in a simple modification of a disposable 5-mL syringe that allows insertion into a common centrifuge and positioning of the syringe on the centrifuge so the PRP separates next to the tip of the syringe. Platelet-derived growth factor BB in PRP was measured under anticoagulant dextrose solution A (ACD-A) or heparin as anticoagulant and with or without prostaglandin E1 (PGE1) as a platelet aggregation suppressant.

RESULTS

The new method successfully created PRP with high platelet-derived growth factor BB in all conditions, and the highest value was obtained by using ACD-A and PGE1.

CONCLUSIONS

The new method is useful, and the use of ACD-A and PGE1 is the most recommended.

背景

尽管富血小板血浆（PRP）如今在包括整容手术或皮肤科在内的各个医学领域是一种常用方法，但处理试剂盒的昂贵价格仍是一个障碍。

方法

报道了一种新型独特的制备PRP的经济方法。该方法包括对一次性5毫升注射器进行简单改造，使其能够插入普通离心机并在离心机上定位，从而使PRP在注射器尖端附近分离。在抗凝葡萄糖溶液A（ACD-A）或肝素作为抗凝剂以及有或没有前列腺素E1（PGE1）作为血小板聚集抑制剂的情况下，对PRP中的血小板衍生生长因子BB进行了测量。