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一种制备富血小板血浆的新经济方法。

A New Economic Method for Preparing Platelet-rich Plasma.

作者信息

Fukaya Mototsugu, Ito Asako

机构信息

Tsurumai Kouen Clinic, Nagoya, Japan.

出版信息

Plast Reconstr Surg Glob Open. 2014 Jul 9;2(6):e162. doi: 10.1097/GOX.0000000000000109. eCollection 2014 Jun.

DOI:10.1097/GOX.0000000000000109
PMID:25289355
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4174234/
Abstract

BACKGROUND

Although platelet-rich plasma (PRP) is nowadays a common method in various medical fields, including cosmetic surgery or dermatology, the expensiveness of the kit for processing is still a hurdle.

METHODS

A new unique economic method for preparing PRP was reported. The method consists in a simple modification of a disposable 5-mL syringe that allows insertion into a common centrifuge and positioning of the syringe on the centrifuge so the PRP separates next to the tip of the syringe. Platelet-derived growth factor BB in PRP was measured under anticoagulant dextrose solution A (ACD-A) or heparin as anticoagulant and with or without prostaglandin E1 (PGE1) as a platelet aggregation suppressant.

RESULTS

The new method successfully created PRP with high platelet-derived growth factor BB in all conditions, and the highest value was obtained by using ACD-A and PGE1.

CONCLUSIONS

The new method is useful, and the use of ACD-A and PGE1 is the most recommended.

摘要

背景

尽管富血小板血浆（PRP）如今在包括整容手术或皮肤科在内的各个医学领域是一种常用方法，但处理试剂盒的昂贵价格仍是一个障碍。

方法

报道了一种新型独特的制备PRP的经济方法。该方法包括对一次性5毫升注射器进行简单改造，使其能够插入普通离心机并在离心机上定位，从而使PRP在注射器尖端附近分离。在抗凝葡萄糖溶液A（ACD-A）或肝素作为抗凝剂以及有或没有前列腺素E1（PGE1）作为血小板聚集抑制剂的情况下，对PRP中的血小板衍生生长因子BB进行了测量。

结果

新方法在所有条件下均成功制备出具有高血小板衍生生长因子BB的PRP，使用ACD-A和PGE1时获得的值最高。

结论

新方法有用，最推荐使用ACD-A和PGE1。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/44f6988dfe49/gox-2-e162-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/6a3b9897c036/gox-2-e162-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/5ed92a1b45d9/gox-2-e162-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/1344c0cf748f/gox-2-e162-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/c0df81e07c8c/gox-2-e162-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/969fac7ecd9d/gox-2-e162-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/ff57f5106a63/gox-2-e162-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/322c94b05587/gox-2-e162-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/44f6988dfe49/gox-2-e162-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/6a3b9897c036/gox-2-e162-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/5ed92a1b45d9/gox-2-e162-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/1344c0cf748f/gox-2-e162-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/c0df81e07c8c/gox-2-e162-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/969fac7ecd9d/gox-2-e162-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/ff57f5106a63/gox-2-e162-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/322c94b05587/gox-2-e162-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecd/4174234/44f6988dfe49/gox-2-e162-g009.jpg

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