Rathi Bharathi, Devanesan Sandhanasamy, AlSalhi Mohamad S, Ranjith Singh Amirtham J
Department of Biochemistry, Shrimati Indira Gandhi College, Trichy, Tamil Nadu, India.
Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box, 2455, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2021 Mar;28(3):1576-1581. doi: 10.1016/j.sjbs.2020.12.051. Epub 2021 Jan 5.
The antioxidant potential and antiproliferative activity of the extracts of seeds (Black Cummins) and honey formulations are to be explored.
The gas chromatography-mass spectrum (GC-MS) and Thin Layer Chromatography (TLC) fingerprint o and Honey formulation revealed alkaloid, saponin, volatile oil, flavonoid, glycosides, sugar, and phenolic compound in the extract. GC-MS profiling of the cold extract of seeds and honey formulation shows peaks for eleven fractions of compounds. Using TLC, the phenolic compounds of and honey formulations were separated.
The current study discovers the cytotoxic effect of black Cummins seeds and honey formulation on human ovarian cancer (PA-1) cell line as assessed by MTT assay. PA-1 cells were inhibited with the increasing concentration of seeds extract and honey formulation.
The study validates the importance of the tested extracts in the treatment of cancer.
探索种子（黑孜然）提取物和蜂蜜配方的抗氧化潜力及抗增殖活性。
气相色谱 - 质谱联用（GC - MS）和薄层色谱（TLC）对种子和蜂蜜配方进行指纹图谱分析，结果显示提取物中含有生物碱、皂苷、挥发油、黄酮类、糖苷、糖和酚类化合物。种子冷提取物和蜂蜜配方的GC - MS分析显示有11个化合物馏分的峰。通过TLC分离了种子和蜂蜜配方中的酚类化合物。
通过MTT法评估，本研究发现黑孜然种子和蜂蜜配方对人卵巢癌（PA - 1）细胞系具有细胞毒性作用。随着种子提取物和蜂蜜配方浓度的增加，PA - 1细胞受到抑制。
该研究证实了所测试提取物在癌症治疗中的重要性。
