The Ken and Ruth Davee Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
STAR Protoc. 2021 Mar 6;2(1):100372. doi: 10.1016/j.xpro.2021.100372. eCollection 2021 Mar 19.
The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein. For complete details on the use and execution of this protocol, please refer to Cuddy et al. (2019) and Zunke et al. (2018).
蛋白质聚集成不溶性聚集体是几种神经退行性疾病的共同特征。聚集的α-突触核蛋白是路易体的主要成分，路易体病理性地定义了帕金森病（PD）。在这里，我们介绍了在诱导多能干细胞（iPSC）患者来源的神经元模型和脑组织中检测α-突触核蛋白致病构象的方法。这些方法可用于研究 PD 的发病机制和发现恢复生理α-突触核蛋白的新型治疗方法。有关该方案使用和执行的完整详细信息，请参阅 Cuddy 等人（2019 年）和 Zunke 等人（2018 年）。
