Departments of Cardiology and Pathology, Smidt Heart Institute, Advanced Clinical BioSystems Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA.
Circ Res. 2021 Mar 19;128(6):720-722. doi: 10.1161/CIRCRESAHA.121.318922. Epub 2021 Mar 18.
Protein isoforms have highly similar amino acid sequences which can lead to similar biological roles. However in some cases, such as cell specific or enriched isoforms, they can assume distinct functions. This editorial discusses the work by Peper that defined the novel cardiac-interactome of caveolin 3 and the downstream functional consequences. Proteins live in a closely controlled communities where their complexes are highly regulated as are their location within a cell. There is a suite of ever-growing proteomic technologies capable of quantifying the chemical and biological diversity of the proteome including protein interactomes. This editorial discusses cutting-edge spatial proteomic technologies able to measure and define protein interactomes within living cells and precisely explore the immense landscape of the proteome.
蛋白质异构体具有高度相似的氨基酸序列，这可能导致它们具有相似的生物学功能。然而，在某些情况下，如细胞特异性或富集的异构体，它们可能具有不同的功能。这篇社论讨论了 Peper 的工作，该工作定义了 caveolin 3 的新型心脏相互作用组及其下游功能后果。蛋白质生活在一个受到严格控制的环境中，它们的复合物受到高度调节，就像它们在细胞内的位置一样。有一系列不断发展的蛋白质组学技术能够定量测量蛋白质组的化学和生物学多样性，包括蛋白质相互作用组。这篇社论讨论了前沿的空间蛋白质组学技术，这些技术能够测量和定义活细胞内的蛋白质相互作用组，并精确探索蛋白质组的巨大景观。
