Kibar Hazal, Arslan Yavuz Emre, Ceylan Ahmet, Karaca Başar, Haliscelik Ozan, Kiran Fadime
Pharmabiotic Technologies Research Laboratory, Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Turkey; Regenerative Biomaterials Laboratory, Department of Bioengineering, Faculty of Engineering, Canakkale Onsekiz Mart University, 17100 Canakkale, Turkey.
Regenerative Biomaterials Laboratory, Department of Bioengineering, Faculty of Engineering, Canakkale Onsekiz Mart University, 17100 Canakkale, Turkey.
Int J Biol Macromol. 2020 Dec 15;165(Pt B):2900-2908. doi: 10.1016/j.ijbiomac.2020.10.106. Epub 2020 Oct 22.
Healing and regeneration of periodontium are considered as a complex physiological process. Therefore, treatments need to be addressed with highly effective components modulating the multiple pathways. In this study, exopolysaccharide (EPS) produced by Weissella cibaria EIR/P2, was partially purified from the culture supernatant and subjected to characterization within the aim of evaluating its potential for periodontal regeneration. High-Performance Liquid Chromatography analysis revealed a single-peak corresponding to the glucose which identified the EPS as dextran. Fourier transform-infrared spectra were also displayed characteristic peaks for polysaccharides. According to the results of gel permeation/size exclusion-chromatography, the molecular mass was determined to be 8 × 10 Da. To clarify its anti-bacterial activity on Streptococcus mutans, effects on viability and biofilm formation was evaluated. At 50 mg/mL, dextran exhibited a bactericidal effect with 70% inhibition on biofilm formation. Besides, dose-dependent antioxidant effects were also detected. The efficacy of dextran in enhancing the viability of human periodontal ligament fibroblast cells (hPDLFCs) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay, and an increase was observed in the viability of hPDLFCs. In conclusion, dextran derived from W. cibaria can be potentially used as a multi-functional bioactive polymer in the design of new therapeutic strategies to promote healing and regeneration of periodontium.
牙周组织的愈合与再生被认为是一个复杂的生理过程。因此，治疗需要使用能够调节多种途径的高效成分来进行。在本研究中，从巴氏魏斯氏菌EIR/P2产生的胞外多糖（EPS）从培养上清液中部分纯化，并进行表征，目的是评估其在牙周再生方面的潜力。高效液相色谱分析显示出一个对应于葡萄糖的单峰，这表明该EPS为葡聚糖。傅里叶变换红外光谱也显示出多糖的特征峰。根据凝胶渗透/尺寸排阻色谱的结果，确定其分子量为8×10 Da。为了阐明其对变形链球菌的抗菌活性，评估了其对细胞活力和生物膜形成的影响。在50 mg/mL时，葡聚糖表现出杀菌作用，对生物膜形成的抑制率为70%。此外，还检测到了剂量依赖性的抗氧化作用。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估了葡聚糖增强人牙周膜成纤维细胞（hPDLFCs）活力的功效，观察到hPDLFCs的活力有所增加。总之，来源于巴氏魏斯氏菌的葡聚糖在设计促进牙周组织愈合和再生的新治疗策略中具有潜在的多功能生物活性聚合物的用途。
