Krebs Fanny S, Zoete Vincent, Trottet Maxence, Pouchon Timothée, Bovigny Christophe, Michielin Olivier
Computer-Aided Molecular Engineering, Department of Oncology, Ludwig Institute for Cancer Research Lausanne Branch, University of Lausanne, Lausanne, Switzerland.
Molecular Modelling Group, Swiss Institute of Bioinformatics (SIB), Lausanne, Switzerland.
NPJ Precis Oncol. 2021 Mar 18;5(1):19. doi: 10.1038/s41698-021-00156-5.
Swiss-PO is a new web tool to map gene mutations on the 3D structure of corresponding proteins and to intuitively assess the structural implications of protein variants for precision oncology. Swiss-PO is constructed around a manually curated database of 3D structures, variant annotations, and sequence alignments, for a list of 50 genes taken from the Ion AmpliSeq Custom Cancer Hotspot Panel. The website was designed to guide users in the choice of the most appropriate structure to analyze regarding the mutated residue, the role of the protein domain it belongs to, or the drug that could be selected to treat the patient. The importance of the mutated residue for the structure and activity of the protein can be assessed based on the molecular interactions exchanged with neighbor residues in 3D within the same protein or between different biomacromolecules, its conservation in orthologs, or the known effect of reported mutations in its 3D or sequence-based vicinity. Swiss-PO is available free of charge or login at https://www.swiss-po.ch.
Swiss-PO是一种新的网络工具，用于在相应蛋白质的三维结构上绘制基因突变，并直观地评估蛋白质变体对精准肿瘤学的结构影响。Swiss-PO围绕一个人工策划的数据库构建，该数据库包含三维结构、变体注释和序列比对，涉及从Ion AmpliSeq定制癌症热点面板中选取的50个基因列表。该网站旨在指导用户选择最合适的结构进行分析，具体可根据突变残基、其所属蛋白质结构域的作用或可选择用于治疗患者的药物。可以基于与同一蛋白质内或不同生物大分子之间三维空间中的相邻残基交换的分子相互作用、其在直系同源物中的保守性，或其三维或基于序列的邻近区域中报道突变的已知效应，来评估突变残基对蛋白质结构和活性的重要性。Swiss-PO可免费使用，登录网址为https://www.swiss-po.ch。
