Prieto Diego, González Camila, Weber Laura, Realini Ornella, Pino-Lagos Karina, Bendek Maria José, Retamal Ignacio, Beltrán Víctor, Riedemann Juan Pablo, Espinoza Francisco, Chaparro Alejandra
Department of Periodontology, Center for Biomedical and Innovation Research, Laboratory of Periodontal Research, Faculty of Dentistry, Universidad de los Andes, Santiago, Chile.
Centro de Investigación e Innovación Biomédica, Facultad de Medicina, Universidad de los Andes, Santiago, Chile.
J Oral Biol Craniofac Res. 2021 Apr-Jun;11(2):303-307. doi: 10.1016/j.jobcr.2021.02.010. Epub 2021 Feb 22.
To explore the soluble Neuropilin-1 (sNRP-1) concentrations in gingival crevicular fluid (GCF) and the periodontal clinical status of patients with Rheumatoid Arthritis (RA).
We conducted an exploratory study with 40 study participants, 20 with RA, and 20 healthy controls. Clinical and periodontal data were recorded, and GCF samples were obtained. sNRP-1 levels in GCF were determined by ELISA assay. Descriptive statistics, Mann-Whitney test, Unpaired -test, logistic regression model, and Area Under Receiver Operating Characteristic Curve (AUC-ROC) were made to explore the diagnostic performance accuracy.
RA patients had significantly higher levels of sNRP-1 in GCF (p = 0.0447). The median levels of GCF-sNRP-1 were 208.85 pg/μl (IQR 131.03) in the RA group compared to 81.46 pg/μl (IQR 163.73) in the control group. We observed an association between the GCF-sNRP-1 concentrations and the RA diagnosis (OR:1.009; CI 1.00-1.001; p = 0.047). The diagnosis of chronic periodontitis was also associated with RA (OR: 6.9; CI 1.52-31.37; p = 0.012). Moreover, the AUC-ROC of GCF-sNRP-1 concentrations combined with periodontal clinical parameters such as periodontal probing depth and periodontal inflamed surface area was 0.80.
This exploratory case-control study shows that RA patients had significantly higher levels of sNRP-1 in GCF. New longitudinal studies are necessary to evaluate the role of NRP-1 in periodontal tissues and consider it an oral biomarker with clinical value in RA.
探讨类风湿关节炎（RA）患者龈沟液（GCF）中可溶性神经纤毛蛋白-1（sNRP-1）的浓度及牙周临床状况。
我们对40名研究参与者进行了一项探索性研究，其中20名RA患者和20名健康对照者。记录临床和牙周数据，并获取GCF样本。通过酶联免疫吸附测定法（ELISA）测定GCF中的sNRP-1水平。采用描述性统计、曼-惠特尼检验、非配对t检验、逻辑回归模型和受试者操作特征曲线下面积（AUC-ROC）来探索诊断性能准确性。
RA患者GCF中sNRP-1水平显著更高（p = 0.0447）。RA组GCF-sNRP-1的中位数水平为208.85 pg/μl（四分位间距131.03），而对照组为81.46 pg/μl（四分位间距163.73）。我们观察到GCF-sNRP-1浓度与RA诊断之间存在关联（比值比：1.009；可信区间1.00 - 1.001；p = 0.047）。慢性牙周炎的诊断也与RA相关（比值比：6.9；可信区间1.52 - 31.37；p = 0.012）。此外，GCF-sNRP-1浓度与牙周探诊深度和牙周炎症表面积等牙周临床参数相结合的AUC-ROC为0.80。
这项探索性病例对照研究表明，RA患者GCF中sNRP-1水平显著更高。有必要开展新的纵向研究来评估NRP-1在牙周组织中的作用，并将其视为RA中有临床价值的口腔生物标志物。
