Saberianpour Shirin, Rahbarghazi Reza, Ahmadi Mahdi, Nouri Mohammad, Heidarzadeh Morteza, Karimi Abbas, Nemati Souror
Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2021 Jan;11(1):163-170. doi: 10.34172/apb.2021.017. Epub 2020 Nov 7.
Here, we investigated the angiogenic potential of endothelial progenitor cells juxtaposed with mesenchymal stem cells (MSCs) inside alginate-gelatin microspheres with stromal derived factor-1α (SDF-1 α) for 7 days. Six encapsulated groups were allocated including endothelial progenitor cells (EPCs), EPCs/SDF-1α, MSCs, MSCs/SDF-1α, EPCs+MSCs and EPCs+MSCs/SDF-1α. Cells were encapsulated with a mixture of 1% alginate and 2% gelatin hydrogel. Cell survival was examined by MTT assay. Endothelial differentiation was determined by flow cytometry and ELISA. Tubulogenesis assay and Ac-Dil-LDL uptake were used to detect functional activity. Cell migration was analyzed by Transwell insert and gelatin zymography analyses. By using real-time polymerase chain reaction (PCR), we measured the transcription of and . We found an increase in cell viability in MSCs/SDF-1α microspheres compared to EPCs group ( <0.05). EPC/MSCs co-culture contributed to the increase of CD133+ cells while we found high CD31 levels in MSCs group ( <0.05). Juxtaposition of EPC with MSCs increased tubulogenesis compared to SDF-1a-free condition ( <0.001). SDF-1α had the potential to increase in AC-LDL uptake in MSCs and EPCs/MSCs groups. Cells migration and MMP-9 activities increased after treatment with SDF-1α. SDF-1α upregulated and in encapsulated cells, especially in a co-culture system. Alginate-gelatin microspheres could alter the angiogenic potential of progenitor cells in the presence of SDF-1α.
在此，我们研究了内皮祖细胞与间充质干细胞（MSCs）在含有基质细胞衍生因子-1α（SDF-1α）的藻酸盐-明胶微球内共培养7天的血管生成潜力。共设置了6个包封组，包括内皮祖细胞（EPCs）、EPCs/SDF-1α、MSCs、MSCs/SDF-1α、EPCs+MSCs和EPCs+MSCs/SDF-1α。细胞用1%藻酸盐和2%明胶水凝胶的混合物进行包封。通过MTT法检测细胞存活率。通过流式细胞术和酶联免疫吸附测定法测定内皮分化。采用成管试验和乙酰化低密度脂蛋白摄取检测功能活性。通过Transwell小室和明胶酶谱分析来分析细胞迁移。通过实时聚合酶链反应（PCR），我们检测了[具体基因名称未给出]和[具体基因名称未给出]的转录情况。我们发现，与EPCs组相比，MSCs/SDF-1α微球中的细胞活力有所增加（P<0.05）。EPC/MSCs共培养导致CD133+细胞增加，而我们在MSCs组中发现CD31水平较高（P<0.05）。与无SDF-1α的条件相比，EPC与MSCs并列共培养可增加成管能力（P<0.001）。SDF-1α有可能增加MSCs组和EPCs/MSCs组中乙酰化低密度脂蛋白的摄取。用SDF-1α处理后，细胞迁移和基质金属蛋白酶-9（MMP-9）活性增加。SDF-1α上调了包封细胞中的[具体基因名称未给出]和[具体基因名称未给出]，尤其是在共培养系统中。在SDF-1α存在的情况下，藻酸盐-明胶微球可改变祖细胞的血管生成潜力。
