多组学分析揭示了乳制品消费、肠道微生物群和心血管代谢健康之间的关系。

Multi-omics analyses reveal relationships among dairy consumption, gut microbiota and cardiometabolic health.

机构信息

School of Life Sciences, Fudan University, Shanghai, China; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China.

Guangdong Provincial Key Laboratory of Food, Nutrition and Health; Department of Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, China.

出版信息

EBioMedicine. 2021 Apr;66:103284. doi: 10.1016/j.ebiom.2021.103284. Epub 2021 Mar 19.

DOI:10.1016/j.ebiom.2021.103284

PMID:33752125

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7985282/

Abstract

BACKGROUND

Little is known about the interplay among dairy intake, gut microbiota and cardiometabolic health in human prospective cohort studies.

METHODS

The present study included 1780 participants from the Guangzhou Nutrition and Health Study. We examined the prospective association between habitual dairy consumption (total dairy, milk, yogurt) and gut microbial composition using linear regression after adjusting for socio-demographic, lifestyle and dietary factors. The cross-sectional association of dairy-associated microbial features with cardiometabolic risk factors was examined with a linear regression model, adjusting for potential confounders. Serum metabolomic profiles were analyzed by partial correlation analysis.

FINDINGS

There was a significant overall difference in gut microbial community structure (β-diversity) comparing the highest with the lowest category for each of total dairy, milk and yogurt (P < 0.05). We observed that dairy-associated microbes and α-diversity indices were inversely associated with blood triglycerides, while positively associated with high-density lipoprotein cholesterol. A follow-up metabolomics analysis revealed the association of targeted serum metabolites with dairy-microbial features and cardiometabolic traits. Specifically, 2-hydroxy-3-methylbutyric acid, 2-hydroxybutyric acid and L-alanine were inversely associated with dairy-microbial score, while positively associated with triglycerides (FDR-corrected P < 0.1).

INTERPRETATION

Dairy consumption is associated with the gut microbial composition and a higher α-diversity, which provides new insights into the understanding of dairy-gut microbiota interactions and their relationship with cardiometabolic health.

FUNDING

This work was supported by the National Natural Science Foundation of China, Zhejiang Ten-thousand Talents Program, Westlake University and the 5010 Program for Clinical Researches of the Sun Yat-sen University.

摘要

背景

在人类前瞻性队列研究中，关于乳制品摄入、肠道微生物群和心脏代谢健康之间的相互作用知之甚少。

方法

本研究纳入了来自广州营养与健康研究的 1780 名参与者。我们使用线性回归调整了社会人口统计学、生活方式和饮食因素后，研究了习惯性乳制品消费（总乳制品、牛奶、酸奶）与肠道微生物组成之间的前瞻性关联。使用线性回归模型，调整潜在混杂因素后，研究了与乳制品相关的微生物特征与心脏代谢危险因素的横断面关联。通过偏相关分析分析血清代谢组学谱。

结果

总乳制品、牛奶和酸奶中，每个类别的最高和最低类别之间的肠道微生物群落结构（β多样性）存在显著差异（P < 0.05）。我们观察到，与乳制品相关的微生物和α多样性指数与血液甘油三酯呈负相关，而与高密度脂蛋白胆固醇呈正相关。后续的代谢组学分析显示，靶向血清代谢物与乳制品相关的微生物特征和心脏代谢特征之间存在关联。具体而言，2-羟基-3-甲基丁酸、2-羟基丁酸和 L-丙氨酸与乳制品微生物评分呈负相关，而与甘油三酯呈正相关（经 FDR 校正的 P < 0.1）。