1Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia.
2Fundación para la Salud Integral de los Guatemaltecos, FUNSALUD, Quetzaltenango, Guatemala.
Am J Trop Med Hyg. 2021 Mar 22;104(5):1734-1736. doi: 10.4269/ajtmh.20-1497.
Molecular detection of dengue virus (DENV) RNA from serum or plasma provides an accurate acute-phase diagnostic (< 7 days after symptom onset). Detection may be prolonged in whole blood, although data are limited. We tested for DENV by real-time reverse transcription-PCR in 345 paired acute-phase plasma and whole blood samples from individuals with a Flavivirus-like illness in southwestern Guatemala. In 18/18 cases with detectable DENV RNA in plasma, whole blood samples were positive and yielded similar cycle threshold values. In seven individuals with convalescent samples obtained 2-3 weeks later, DENV RNA remained detectable in whole blood but not plasma. In three additional cases, DENV RNA was only detectable in whole blood at the acute visit. In two cases, whole blood detection was linked to a virologically confirmed DENV infection 6-11 weeks earlier. Whole blood DENV RNA detection is sensitive for acute dengue infection and may remain positive for weeks to months.
从血清或血浆中检测登革热病毒 (DENV) RNA 可提供准确的急性诊断（发病后<7 天）。虽然数据有限，但在全血中检测可能会延长。我们在危地马拉西南部患有类似黄病毒病的 345 对急性血浆和全血样本中通过实时逆转录聚合酶链反应 (RT-PCR) 检测 DENV。在 18/18 例血浆中可检测到 DENV RNA 的病例中，全血样本均为阳性且产生相似的循环阈值。在随后 2-3 周获得恢复期样本的 7 名个体中，DENV RNA 仍可在全血中检测到，但在血浆中不可检测到。在另外 3 例中，仅在急性就诊时可在全血中检测到 DENV RNA。在 2 例中，全血检测与 6-11 周前经病毒学确认的 DENV 感染有关。全血 DENV RNA 检测对急性登革热感染敏感，并且可能持续数周到数月呈阳性。
