Grupo de Investigación Biomedicina, Facultad de Medicina, Institución Universitaria Visión de las Américas, Pereira, Risaralda, Colombia.
Emerging Infectious Diseases and Tropical Medicine Research Group, Sci-help, Pereira, Risaralda, Colombia.
Am J Trop Med Hyg. 2022 Jul 5;107(2):296-299. doi: 10.4269/ajtmh.21-1087. Print 2022 Aug 17.
Standard molecular detection of many pathogens, in particular RNA viruses, requires appropriate handling in the field for preserving the quality of the sample until processing. This could be challenging in remote tropical areas. Dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) are RNA viruses, prominent among the causes of fever in the tropics. We aimed to test the stability of arboviral RNA in contrived dried blood spots prepared on Whatman 903 Protein saver cards as a means of sample collection and storage. We were able to detect DENV, CHIKV, and ZIKV by real-time RT-PCR up to 180 days after card inoculation with stable Ct values across the study period. Our study supports dried blood spots (DBS) on protein saver cards as a platform for stable detection of arboviral RNA of sufficient quality to be used in diagnostic RT-PCR assays and next generation sequencing.
标准的分子检测需要对许多病原体进行适当处理，特别是 RNA 病毒，以保持样本的质量直到处理。这在偏远的热带地区可能具有挑战性。登革热病毒（DENV）、基孔肯雅热病毒（CHIKV）和寨卡病毒（ZIKV）是 RNA 病毒，是热带地区发热的主要原因之一。我们旨在测试在沃特曼 903 蛋白保存卡上制备的人为干燥血斑中虫媒病毒 RNA 的稳定性，作为收集和储存样本的一种手段。通过实时 RT-PCR，我们能够在接种卡片后长达 180 天内检测到 DENV、CHIKV 和 ZIKV，整个研究期间 Ct 值稳定。我们的研究支持蛋白保存卡上的干燥血斑（DBS）作为一个平台，用于稳定检测虫媒病毒 RNA，其质量足以用于诊断 RT-PCR 检测和下一代测序。
