Lee Yoona, Son Bokyung, Cha Yoyeon, Ryu Sangryeol
Department of Food and Animal Biotechnology, Seoul National University, Seoul, South Korea.
Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
Front Microbiol. 2021 Mar 8;12:622755. doi: 10.3389/fmicb.2021.622755. eCollection 2021.
is an important human pathogen that can be frequently encountered in clinical and food-processing surroundings. Among the various countermeasures, bacteriophages have been considered to be promising alternatives to antibiotics. In this study, the bacteriophage PALS2 was isolated from bird feces, and the genomic and biological characteristics of this phage were investigated. PALS2 was determined to belong to the family and exhibited extended host inhibition that persisted for up to 24 h with repeated bursts of 12 plaque-forming units/cell. The complete genome of PALS2 measured 268,746 base pairs (bp), indicating that PALS2 could be classified as a jumbo phage. The PALS2 genome contained 279 ORFs and 1 tRNA covering asparagine, and the majority of predicted PALS2 genes encoded hypothetical proteins. Additional genes involved in DNA replication and repair, nucleotide metabolism, and genes encoding multisubunit RNA polymerase were identified in the PALS2 genome, which is a common feature of typical jumbo phages. Comparative genomic analysis indicated that PALS2 is a phiKZ-related virus and is more similar to typical jumbo phages than to staphylococcal phages. Additionally, the effective antimicrobial activities of phage PALS2 suggest its possible use as a biocontrol agent in various clinical and food processing environments.
是一种重要的人类病原体，在临床和食品加工环境中经常遇到。在各种应对措施中，噬菌体被认为是抗生素的有前途的替代品。在本研究中，从鸟类粪便中分离出噬菌体PALS2，并对该噬菌体的基因组和生物学特性进行了研究。PALS2被确定属于 科，并表现出延长的宿主抑制作用，在每次爆发产生12个噬菌斑形成单位/细胞的情况下，这种抑制作用可持续长达24小时。PALS2的完整基因组测量为268,746个碱基对（bp），表明PALS2可被归类为巨型噬菌体。PALS2基因组包含279个开放阅读框（ORF）和1个覆盖天冬酰胺的tRNA，并且大多数预测的PALS2基因编码假定蛋白。在PALS2基因组中鉴定出了与DNA复制和修复、核苷酸代谢有关的其他基因以及编码多亚基RNA聚合酶的基因，这是典型巨型噬菌体的共同特征。比较基因组分析表明，PALS2是一种与phiKZ相关的病毒，与典型巨型噬菌体的相似性高于与葡萄球菌噬菌体的相似性。此外，噬菌体PALS2的有效抗菌活性表明其可能在各种临床和食品加工环境中用作生物控制剂。
