Tang Zhaohui, Tang Ning, Wang Xinwei, Ren Huiying, Zhang Can, Zou Ling, Han Lei, Guo Longzong, Liu Wenhua
College of Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
College of Chemistry and Pharmaceutical Science, Qingdao Agricultural University, Qingdao, Shandong, China.
Front Microbiol. 2023 Feb 16;14:1091442. doi: 10.3389/fmicb.2023.1091442. eCollection 2023.
The high incidence of Avian pathogenic (APEC) in poultry has resulted in significant economic losses. It has become necessary to find alternatives to antibiotics due to the alarming rise in antibiotic resistance. Phage therapy has shown promising results in numerous studies. In the current study, a lytic phage vB_EcoM_CE1 (short for CE1) against () was isolated from broiler feces, showing a relatively wide host range and lysing 56.9% (33/58) of high pathogenic strains of APEC. According to morphological observations and phylogenetic analysis, phage CE1 belongs to the genus, family, containing an icosahedral capsid (80 ~ 100 nm in diameter) and a retractable tail (120 nm in length). This phage was stable below 60°C for 1 h over the pH range of 4 to 10. Whole-genome sequencing revealed that phage CE1 contained a linear double-stranded DNA genome spanning 167,955 bp with a GC content of 35.4%. A total of 271 ORFs and 8 tRNAs were identified. There was no evidence of virulence genes, drug-resistance genes, or lysogeny genes in the genome. The test showed high bactericidal activity of phage CE1 against at a wide range of MOIs, and good air and water disinfectant properties. Phage CE1 showed perfect protection against broilers challenged with APEC strain . This study provides some basic information for further research into treating colibacillosis, or killing in breeding environments.
家禽中禽致病性大肠杆菌（APEC）的高发病率已造成重大经济损失。由于抗生素耐药性惊人上升，寻找抗生素替代品变得十分必要。噬菌体疗法在众多研究中已显示出有前景的结果。在本研究中，从肉鸡粪便中分离出一种针对（此处原文缺失相关内容）的裂解性噬菌体vB_EcoM_CE1（简称CE1），其宿主范围相对较广，能裂解56.9%（33/58）的高致病性APEC菌株。根据形态学观察和系统发育分析，噬菌体CE1属于（此处原文缺失相关内容）属、（此处原文缺失相关内容）科，具有二十面体衣壳（直径80至100纳米）和可收缩尾部（长度120纳米）。该噬菌体在4至10的pH范围内、60°C以下1小时内稳定。全基因组测序表明，噬菌体CE1含有一个线性双链DNA基因组，跨度为167,955碱基对，GC含量为35.4%。共鉴定出271个开放阅读框和8个tRNA。基因组中没有毒力基因、耐药基因或溶原性基因的证据。体外实验表明，噬菌体CE1在广泛的感染复数范围内对（此处原文缺失相关内容）具有高杀菌活性，且具有良好的空气和水消毒性能。噬菌体CE1对受APEC菌株（此处原文缺失相关内容）攻击的肉鸡显示出完美的保护作用。本研究为进一步研究治疗大肠杆菌病或在养殖环境中杀灭（此处原文缺失相关内容）提供了一些基础信息。
