Milani Fabrizia, Muratore Chiara, Biella Sara, Bottoni Martina, Rossi Elio, Colombo Lorenzo, Colombo Paola Sira, Bruschi Piero, Papini Alessio, Landini Paolo, Giuliani Claudia, Araniti Fabrizio, Prinsi Bhakti, Fico Gelsomina
Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Luigi Mangiagalli 25, 20133 Milan, Italy.
"G.E. Ghirardi" Botanical Garden, Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Religione 25, 25088 Toscolano Maderno, Italy.
Plants (Basel). 2025 Jan 23;14(3):340. doi: 10.3390/plants14030340.
A previous ethnobotanical investigation conducted in Valle Imagna (Northern Italy) highlighted the traditional use of L. (Rosaceae) as a disinfectant and wound-healing agent. This use seemed to be linked to a local 18th century manuscript. This species was chosen for a multidisciplinary investigation to validate or refute its traditional use in the valley. Samples from fresh leaves were observed under Scanning Electron and Light Microscopy. The phenolic profiles of an epicuticular aqueous extract of the whole leaves and of infusions and decoctions of leaves and aerial parts were analyzed through Liquid Chromatography-Tandem Mass Spectrometry. The volatile organic compounds (VOCs) of fresh leaves were analyzed through Headspace Solid-Phase Microextraction coupled with Gas Chromatography-Mass Spectrometry. Growth inhibition and adhesion modulation were assessed on , , and by minimum inhibitory concentration and adhesion assays. Two trichome morphotypes were observed: a capitate with a one-celled rounded head and a capitate with a teo-celled cylindrical head. Both were responsible for producing terpenes, while the cylindrical capitates also produced polyphenols. Thirty-four phenolic compounds were characterized. Luteolin-7-O-glucoside, Catechin, and Epicatechin were common to all five extracts. The VOC profiles highlighted the dominance of (+)-α-Pinene. The infusions and the decoctions had a significant inhibitory activity on , and the extracts (specifically, the infusion of the leaves and both decoctions) also had a stimulating effect on the biofilm formation of . These results already hold particular interest because of the strong connection they have to the traditional use of agrimony described in Valle Imagna.
之前在意大利北部伊马尼亚山谷进行的一项民族植物学调查强调了蔷薇科某植物作为消毒剂和伤口愈合剂的传统用途。这种用途似乎与当地一份18世纪的手稿有关。选择该物种进行多学科研究，以验证或反驳其在山谷中的传统用途。对新鲜叶片样本进行了扫描电子显微镜和光学显微镜观察。通过液相色谱 - 串联质谱分析了全叶表皮水性提取物以及叶片和地上部分的浸液和煎剂的酚类概况。通过顶空固相微萃取结合气相色谱 - 质谱分析了新鲜叶片的挥发性有机化合物（VOCs）。通过最低抑菌浓度和黏附试验评估了对[具体菌种1]、[具体菌种2]和[具体菌种3]的生长抑制和黏附调节作用。观察到两种毛状体形态类型：一种是头部为单细胞圆形的头状毛，另一种是头部为双细胞圆柱形的头状毛。两者都负责产生萜类化合物，而圆柱形头状毛还产生多酚。鉴定出34种酚类化合物。木犀草素 - 7 - O - 葡萄糖苷、儿茶素和表儿茶素在所有五种提取物中都有。VOCs图谱突出了（+） - α - 蒎烯的主导地位。浸液和煎剂对[具体菌种1]、[具体菌种2]有显著抑制活性，提取物（特别是叶片浸液和两种煎剂）对[具体菌种3]的生物膜形成也有刺激作用。由于这些结果与伊马尼亚山谷描述的仙鹤草传统用途有紧密联系，已经特别引人关注。
