温度对接种物的影响——琼脂稀释平板计数杀菌测量中潜在的误差来源
Effect of temperature on inoculum as a potential source of error in agar dilution plate count bactericidal measurements.
作者信息
Woolfrey B F, Gresser-Burns M E, Lally R T
机构信息
Department of Anatomic and Clinical Pathology, St. Paul-Ramsey Medical Center, Minnesota 55101.
出版信息
Antimicrob Agents Chemother. 1988 Apr;32(4):513-7. doi: 10.1128/AAC.32.4.513.
Abstract
The effect of increased temperature on Staphylococcus aureus during the inoculation step of the agar dilution plate count method was investigated as a possible cause of artificially high persister counts. For some isolates, exposure of the inoculum to increased temperature resulted in higher persister counts and diminution or loss of the paradoxical effect. The persister patterns for three representative S. aureus isolates are presented to illustrate the strain- and temperature-dependent nature of the phenomenon. For any isolate, the net effect appears to be caused by an interplay of temperature-induced inoculum loss and temperature-induced cell division cycle blockage. A modification of the agar dilution plate count inoculation step to circumvent such problems is described.
摘要
在琼脂稀释平板计数法的接种步骤中，研究了温度升高对金黄色葡萄球菌的影响，这可能是导致人工高持久性菌计数的一个原因。对于一些分离株，接种物暴露于升高的温度会导致更高的持久性菌计数以及反常效应的减弱或丧失。展示了三种代表性金黄色葡萄球菌分离株的持久性菌模式，以说明该现象的菌株和温度依赖性。对于任何分离株，净效应似乎是由温度诱导的接种物损失和温度诱导的细胞分裂周期阻滞相互作用引起的。描述了一种对琼脂稀释平板计数接种步骤的改进方法，以规避此类问题。
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