采用琼脂稀释平板计数法检测金黄色葡萄球菌分离株的苯唑西林杀菌曲线模式。
Oxacillin killing curve patterns of Staphylococcus aureus isolates by agar dilution plate count method.
作者信息
Woolfrey B F, Lally R T, Ederer M N, Gresser-Burns M
出版信息
Antimicrob Agents Chemother. 1987 Jan;31(1):16-20. doi: 10.1128/AAC.31.1.16.
Abstract
The bactericidal dynamics of oxacillin against four Staphylococcus aureus isolates with known 24-h "persister" percentages were studied by using the agar dilution plate count method. Isolates were selected to provide a representative spectrum whose individual 24-h trough intrinsic persister percentages ranged from greater than 1 to less than 0.01%. Resultant agar dilution plate count method killing curve patterns were found to be reproducible and served to characterize each isolate. The paradoxical effect was observed for each isolate, with paradoxical peaks tending to develop and diminish sequentially during the course of oxacillin action. The observed strain-dependent dynamics of oxacillin killing underscore the artifactual nature of the so-called tolerance phenomenon and negate the usefulness of MBCs and MBC/MIC ratios for the characterization of S. aureus isolates.
摘要
采用琼脂稀释平板计数法研究了苯唑西林对4株已知24小时“持留菌”百分比的金黄色葡萄球菌分离株的杀菌动力学。选择分离株以提供一个代表性谱，其个体24小时谷值固有持留菌百分比范围从大于1%到小于0.01%。结果发现，由此产生的琼脂稀释平板计数法的杀灭曲线模式具有可重复性，并可用于表征每个分离株。在苯唑西林作用过程中，每个分离株均观察到了矛盾效应，矛盾峰倾向于依次出现和消失。观察到的苯唑西林杀菌的菌株依赖性动力学强调了所谓耐受性现象的人为性质，并否定了MBCs和MBC/MIC比值用于表征金黄色葡萄球菌分离株的有用性。
相似文献
1
Oxacillin killing curve patterns of Staphylococcus aureus isolates by agar dilution plate count method.采用琼脂稀释平板计数法检测金黄色葡萄球菌分离株的苯唑西林杀菌曲线模式。
Antimicrob Agents Chemother. 1987 Jan;31(1):16-20. doi: 10.1128/AAC.31.1.16.
2
Evaluation of oxacillin tolerance in Staphylococcus aureus by a novel method.用一种新方法评估金黄色葡萄球菌对苯唑西林的耐受性。
Antimicrob Agents Chemother. 1985 Sep;28(3):381-8. doi: 10.1128/AAC.28.3.381.
3
Ampicillin killing curve patterns of Haemophilus influenzae type b isolates by agar dilution plate count method.采用琼脂稀释平板计数法检测b型流感嗜血杆菌分离株的氨苄西林杀菌曲线模式。
Antimicrob Agents Chemother. 1987 Nov;31(11):1711-7. doi: 10.1128/AAC.31.11.1711.
4
Influence of technical factor variations during inoculum preparation on the agar dilution plate-count method for quantitation of Staphylococcus aureus oxacillin persisters.接种物制备过程中技术因素变化对金黄色葡萄球菌苯唑西林持留菌定量琼脂稀释平板计数法的影响。
Antimicrob Agents Chemother. 1986 Nov;30(5):792-3. doi: 10.1128/AAC.30.5.792.
5
Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp.金黄色葡萄球菌和凝固酶阴性葡萄球菌临床分离株中，用于确定苯唑西林（甲氧西林）耐药性的药敏试验方法与mecA基因分析的比较
J Clin Microbiol. 1999 Sep;37(9):2952-61. doi: 10.1128/JCM.37.9.2952-2961.1999.
6
Effect of temperature on inoculum as a potential source of error in agar dilution plate count bactericidal measurements.温度对接种物的影响——琼脂稀释平板计数杀菌测量中潜在的误差来源
Antimicrob Agents Chemother. 1988 Apr;32(4):513-7. doi: 10.1128/AAC.32.4.513.
7
Comparison of tube dilution and microtitre methods for the detection of antibiotic tolerance in strains of Staphylococcus aureus.
J Antimicrob Chemother. 1984 May;13(5):417-21. doi: 10.1093/jac/13.5.417.
8
Agar dilution and agar screen with cefoxitin and oxacillin: what is known and what is unknown in detection of meticillin-resistant Staphylococcus aureus.使用头孢西丁和苯唑西林进行琼脂稀释法和琼脂筛选法：耐甲氧西林金黄色葡萄球菌检测中的已知与未知情况
J Med Microbiol. 2008 Aug;57(Pt 8):954-956. doi: 10.1099/jmm.0.46992-0.
9
Oxacillin, cephalothin, and vancomycin tube macrodilution MBC result reproducibility and equivalence to MIC results for methicillin-susceptible and reputedly tolerant Staphylococcus aureus isolates.苯唑西林、头孢噻吩和万古霉素试管法宏量稀释法测定耐甲氧西林金黄色葡萄球菌和据报道耐受性菌株的最低杀菌浓度（MBC）结果的可重复性及其与最低抑菌浓度（MIC）结果的等效性
Antimicrob Agents Chemother. 1988 Mar;32(3):374-7. doi: 10.1128/AAC.32.3.374.
10
Bactericidal activity of oxacillin against beta-lactamase-hyperproducing Staphylococcus aureus.苯唑西林对超产β-内酰胺酶金黄色葡萄球菌的杀菌活性。
Antimicrob Agents Chemother. 1988 Nov;32(11):1614-8. doi: 10.1128/AAC.32.11.1614.
引用本文的文献
1
Comparison of two techniques for measurement of in vitro killing kinetics of five antibiotics against Pseudomonas aeruginosa.两种测量五种抗生素对铜绿假单胞菌体外杀菌动力学技术的比较。
Eur J Clin Microbiol Infect Dis. 1994 May;13(5):412-7. doi: 10.1007/BF01972000.
2
Ampicillin killing curve patterns of Haemophilus influenzae type b isolates by agar dilution plate count method.采用琼脂稀释平板计数法检测b型流感嗜血杆菌分离株的氨苄西林杀菌曲线模式。
Antimicrob Agents Chemother. 1987 Nov;31(11):1711-7. doi: 10.1128/AAC.31.11.1711.
3
Effect of temperature on inoculum as a potential source of error in agar dilution plate count bactericidal measurements.温度对接种物的影响——琼脂稀释平板计数杀菌测量中潜在的误差来源
Antimicrob Agents Chemother. 1988 Apr;32(4):513-7. doi: 10.1128/AAC.32.4.513.
4
Oxacillin, cephalothin, and vancomycin tube macrodilution MBC result reproducibility and equivalence to MIC results for methicillin-susceptible and reputedly tolerant Staphylococcus aureus isolates.苯唑西林、头孢噻吩和万古霉素试管法宏量稀释法测定耐甲氧西林金黄色葡萄球菌和据报道耐受性菌株的最低杀菌浓度（MBC）结果的可重复性及其与最低抑菌浓度（MIC）结果的等效性
Antimicrob Agents Chemother. 1988 Mar;32(3):374-7. doi: 10.1128/AAC.32.3.374.
5
Macrodilution MBC result reproducibility for methicillin-susceptible and reputedly tolerant Staphylococcus aureus isolates.甲氧西林敏感及据称耐受性金黄色葡萄球菌分离株的宏稀释法最低杀菌浓度结果的可重复性
Antimicrob Agents Chemother. 1988 Sep;32(9):1464. doi: 10.1128/AAC.32.9.1464.
6
Tolerance to penicillin in streptococci of viridans group.草绿色链球菌对青霉素的耐受性
J Clin Pathol. 1989 Sep;42(9):1005-6. doi: 10.1136/jcp.42.9.1005.
7
Tests for bactericidal effects of antimicrobial agents: technical performance and clinical relevance.抗菌剂杀菌效果测试：技术性能与临床相关性
Clin Microbiol Rev. 1992 Oct;5(4):420-32. doi: 10.1128/CMR.5.4.420.
本文引用的文献
1
Paradoxical effect of antibiotics. I. The 'Eagle effect'.抗生素的矛盾效应。I. “伊格尔效应”。
J Antimicrob Chemother. 1982 Oct;10(4):259-60. doi: 10.1093/jac/10.4.259.
2
Determination of minimum bactericidal concentrations of oxacillin for Staphylococcus aureus: influence and significance of technical factors.金黄色葡萄球菌对苯唑西林最低杀菌浓度的测定：技术因素的影响及意义
Antimicrob Agents Chemother. 1983 Jan;23(1):142-50. doi: 10.1128/AAC.23.1.142.
3
Laboratory tests for defining bactericidal activity as predictors of antibiotic efficacy in the treatment of endocarditis due to Staphylococcus aureus in rabbits.用于确定杀菌活性作为兔金黄色葡萄球菌性心内膜炎治疗中抗生素疗效预测指标的实验室检测。
J Infect Dis. 1984 Jun;149(6):904-12. doi: 10.1093/infdis/149.6.904.
4
Paradoxic and tolerant effects of moxalactam on Staphylococcus aureus.
J Infect Dis. 1981 Aug;144(2):178. doi: 10.1093/infdis/144.2.178.
5
Detection of penicillin tolerance in streptococci.链球菌中青霉素耐受性的检测。
J Clin Pathol. 1983 Dec;36(12):1353-6. doi: 10.1136/jcp.36.12.1353.
6
Evaluation of oxacillin tolerance in Staphylococcus aureus by a novel method.用一种新方法评估金黄色葡萄球菌对苯唑西林的耐受性。
Antimicrob Agents Chemother. 1985 Sep;28(3):381-8. doi: 10.1128/AAC.28.3.381.
7
Susceptibility of clinical isolates of Staphylococcus aureus to killing by oxacillin.
Can J Microbiol. 1975 Nov;21(11):1692-7. doi: 10.1139/m75-248.
8
Qualitative study of paradoxical zone phenomenon of penicillins against 17 bacterial species of clinical importance.
Chemotherapy. 1978;24(2):92-6. doi: 10.1159/000237766.
Zotero 插件