用一种新方法评估金黄色葡萄球菌对苯唑西林的耐受性。
Evaluation of oxacillin tolerance in Staphylococcus aureus by a novel method.
作者信息
Woolfrey B F, Lally R T, Ederer M N
出版信息
Antimicrob Agents Chemother. 1985 Sep;28(3):381-8. doi: 10.1128/AAC.28.3.381.
Abstract
A novel agar dilution plate-count procedure for the quantitative measurement of bacterial inhibition and killing is described. For Staphylococcus aureus versus oxacillin, by the agar dilution plate-count procedure it was found that only 1 of 20 clinical isolates and 1 of 7 allegedly tolerant reference isolates met the conventional definition of tolerance. By using inocula of 10(5) CFU per plate, most isolates were demonstrated to have subpopulations of cells which, although inhibited, persisted for 24 h in concentrations significantly above their MICs. The persister percentages at 24 h appeared to be strain dependent, and all persisters exhibited the paradoxical effect. For each isolate, the persister percentage markedly decreased after action by oxacillin for 48 h, and the paradoxical effect was greatly diminished. Our findings suggest that tolerance is an artificial and arbitrary concept that does not adequately characterize the inhibition and killing dynamics associated with the persister phenomenon.
摘要
描述了一种用于定量测量细菌抑制和杀灭的新型琼脂稀释平板计数法。对于金黄色葡萄球菌与苯唑西林，通过琼脂稀释平板计数法发现，20株临床分离株中只有1株以及7株所谓的耐受性参考分离株中只有1株符合传统的耐受性定义。通过在每个平板接种10(5) CFU的接种物，发现大多数分离株具有细胞亚群，这些细胞亚群虽然受到抑制，但在浓度显著高于其最低抑菌浓度（MIC）的情况下仍能持续24小时。24小时时的持续菌百分比似乎取决于菌株，并且所有持续菌都表现出矛盾效应。对于每个分离株，在苯唑西林作用48小时后，持续菌百分比显著下降，矛盾效应也大大减弱。我们的研究结果表明，耐受性是一个人为且随意的概念，不足以充分描述与持续菌现象相关的抑制和杀灭动态。
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