Osman Noha M, Shafey Heba I, Abdelhafez Mohamed A, Sallam Ahmed M, Mahrous Karima F
Department of Cell Biology, National Research Centre, El Buhouth Street, 12311, Dokki, Egypt.
Animal and Poultry Production Division, Desert Research Center, 11753, Mataryia, Egypt.
Vet World. 2021 Feb;14(2):475-482. doi: 10.14202/vetworld.2021.475-482. Epub 2021 Feb 23.
Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. () plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study sought to investigate genetic variation in the first intron of the gene and the association of variants with growth traits in major sheep breeds in Egypt (Barki, Ossimi, and Rahmani) and Saudi Arabia (Najdi) using polymerase chain reaction (PCR) and sequencing.
Blood samples were collected, and DNA was extracted from 75 animals. A 386 bp fragment in the first intron of the gene was amplified using PCR. Polymorphic sites were detected using direct sequencing and then correlated with growth traits using a general linear model.
Sequence analysis of the first intron of gene identified six single-nucleotide polymorphisms (SNPs) in the studied breeds. Four mutual SNPs were determined: c.18 G>T, c.241 T>C, c.243 G>A, and c.259 G>T. In addition, two SNPs c.159 A>T and c.173 T>G were monomorphic (AA and TT, respectively) in the Ossimi, Rahmani, and Najdi breeds and polymorphic in the Barki breed. The association analysis revealed that the c.18 G>T and c.241 C>T significantly associated (p<0.05) with birth weight and average daily weight gain, respectively.
Our results strongly support as a candidate gene for marker-assisted selection in sheep breeding programs. Furthermore, the identified variants may be considered as putative markers to improve growth traits in sheep.
在发展中国家，绵羊的生产力至关重要，因为这种动物是肉类和羊毛的重要来源。（）通过调节肌肉生长、分化和再生，在肌肉量的调节中发挥重要作用。本研究旨在利用聚合酶链反应（PCR）和测序技术，研究埃及主要绵羊品种（巴尔基、奥西米和拉赫曼尼）和沙特阿拉伯（纳吉迪）中该基因第一内含子的遗传变异以及变异与生长性状的关联。
采集血液样本，从75只动物中提取DNA。使用PCR扩增该基因第一内含子中的一个386 bp片段。通过直接测序检测多态性位点，然后使用一般线性模型将其与生长性状相关联。
该基因第一内含子的序列分析在研究的品种中鉴定出六个单核苷酸多态性（SNP）。确定了四个共同的SNP：c.18 G>T、c.241 T>C、c.243 G>A和c.259 G>T。此外，两个SNP c.159 A>T和c.173 T>G在奥西米、拉赫曼尼和纳吉迪品种中是单态的（分别为AA和TT），在巴尔基品种中是多态的。关联分析显示，c.18 G>T和c.241 C>T分别与出生体重和平均日增重显著相关（p<0.05）。
我们的结果有力地支持将（）作为绵羊育种计划中标记辅助选择的候选基因。此外，鉴定出的变异可被视为改善绵羊生长性状的推定标记。
