Zhang Shuang, Zhou Hong, Kong Na, Wang Zezheng, Fu Huangmei, Zhang Yangheng, Xiao Yin, Yang Wenrong, Yan Fuhua
Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, People's Republic of China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Bioact Mater. 2021 Mar 13;6(10):3288-3299. doi: 10.1016/j.bioactmat.2021.02.035. eCollection 2021 Oct.
Gold nanoparticles (AuNPs) with surface-anchored molecules present tremendous potential in tissue regeneration. However, little is known about chiral-modified AuNPs. In this study, we successfully prepared L/D-cysteine-anchored AuNPs (L/D-Cys-AuNPs) and studied the effects of chiral-modified AuNPs on osteogenic differentiation and autophagy of human periodontal ligament cells (hPDLCs) and periodontal tissue regeneration. , more L-Cys-AuNPs than D-Cys-AuNPs tend to internalize in hPDLCs. L-Cys-AuNPs also significantly increased the expression of alkaline phosphatase, collagen type 1, osteocalcin, runt-related transcription factor 2, and microtubule-associated protein light chain 3 II and decreased the expression of sequestosome 1 in hPDLCs compared to the expression levels in the hPDLCs treated by D-Cys-AuNPs. tests in a rat periodontal-defect model showed that L-Cys-AuNPs had the greatest effect on periodontal-tissue regeneration. The activation of autophagy in L-Cys-AuNP-treated hPDLCs may be responsible for the cell differentiation and tissue regeneration. Therefore, compared to D-Cys-AuNPs, L-Cys-AuNPs show a better performance in cellular internalization, regulation of autophagy, cell osteogenic differentiation, and periodontal tissue regeneration. This demonstrates the immense potential of L-Cys-AuNPs for periodontal regeneration and provides a new insight into chirally modified bioactive nanomaterials.
表面锚定分子的金纳米颗粒（AuNPs）在组织再生方面具有巨大潜力。然而，关于手性修饰的AuNPs却知之甚少。在本研究中，我们成功制备了L/D-半胱氨酸锚定的AuNPs（L/D-Cys-AuNPs），并研究了手性修饰的AuNPs对人牙周膜细胞（hPDLCs）成骨分化和自噬以及牙周组织再生的影响。与D-Cys-AuNPs相比，更多的L-Cys-AuNPs倾向于被hPDLCs内化。与用D-Cys-AuNPs处理的hPDLCs中的表达水平相比，L-Cys-AuNPs还显著增加了hPDLCs中碱性磷酸酶、I型胶原蛋白、骨钙素、 runt相关转录因子2和微管相关蛋白轻链3 II的表达，并降低了聚集体蛋白1的表达。在大鼠牙周缺损模型中的测试表明，L-Cys-AuNPs对牙周组织再生的影响最大。L-Cys-AuNP处理的hPDLCs中自噬的激活可能是细胞分化和组织再生的原因。因此，与D-Cys-AuNPs相比，L-Cys-AuNPs在细胞内化、自噬调节、细胞成骨分化和牙周组织再生方面表现出更好的性能。这证明了L-Cys-AuNPs在牙周再生方面的巨大潜力，并为手性修饰的生物活性纳米材料提供了新的见解。
