Berrones Reyes Jessica, Kuimova Marina K, Vilar Ramon
Department of Chemistry, Imperial College London, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.
Department of Chemistry, Imperial College London, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.
Curr Opin Chem Biol. 2021 Apr;61:179-190. doi: 10.1016/j.cbpa.2021.02.007. Epub 2021 Mar 27.
Transition and lanthanide metal complexes have rich photophysical properties that can be used for cellular imaging, biosensing and phototherapy. One of the applications of such luminescent compounds is the detection and visualisation of nucleic acids. In this brief review, we survey the recent literature on the use of luminescent metal complexes (including Re, Ru, Os, Ir, Pt, Eu and Tb) as DNA optical probes, including examples of compounds that bind selectively to non-duplex DNA topologies such as quadruplex, i-motif and DNA mismatches. We discuss the applications of metal-based luminescent complexes in cellular imaging, including time-resolved microscopy and super-resolution techniques. Their applications in biosensing and phototherapy are briefly mentioned in the relevant sections.
过渡金属和镧系金属配合物具有丰富的光物理性质，可用于细胞成像、生物传感和光疗。这类发光化合物的应用之一是核酸的检测和可视化。在这篇简短的综述中，我们概述了有关使用发光金属配合物（包括铼、钌、锇、铱、铂、铕和铽）作为DNA光学探针的近期文献，包括选择性结合非双链DNA拓扑结构（如四链体、i-基序和DNA错配）的化合物实例。我们讨论了金属基发光配合物在细胞成像中的应用，包括时间分辨显微镜和超分辨率技术。在相关部分简要提及了它们在生物传感和光疗中的应用。
