Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.
J Assist Reprod Genet. 2021 Aug;38(8):2097-2105. doi: 10.1007/s10815-021-02165-6. Epub 2021 Mar 30.
To report experience designing and establishing a reproductive registry and sample biorepository and to describe initial subject characteristics and biospecimens.
Beginning in December 2017, patients presenting for reproductive care at the University of Michigan were approached for study enrollment. Following consent, subjects completed detailed reproductive and health questionnaires. A variety of reproductive specimens and tissues were collected and processed for multiple downstream applications.
Subject enrollment began in December of 2017. There are currently 1798 subjects enrolled. Female participants report a variety of reproductive disorders. Available samples include semen, sperm, follicular fluid, granulosa cells, immature oocytes, ovarian and uterine tissue, and blood samples.
We report the successful establishment of a reproductive registry and sample biorepository. Furthermore, we describe methods for collection and storage of a variety of reproductive tissue processed for multiple downstream translational applications.
报告设计和建立生殖注册和样本生物库的经验，并描述初始研究对象特征和生物样本。
从 2017 年 12 月开始，在密歇根大学接受生殖治疗的患者被招募参与该研究。在获得同意后，研究对象完成了详细的生殖和健康问卷。收集了各种生殖标本和组织，并进行了多种下游应用的处理。
研究对象的招募工作于 2017 年 12 月开始。目前已招募 1798 名研究对象。女性参与者报告了各种生殖障碍。现有的样本包括精液、精子、卵泡液、颗粒细胞、不成熟卵母细胞、卵巢和子宫组织以及血样。
我们报告了成功建立生殖注册和样本生物库。此外，我们还描述了用于多种下游转化应用的多种生殖组织的收集和存储方法。
