Rajyalakshmi B, Samavedam Srinivas, Reddy P Ramakrishna, Aluru Narmada
Department of Critical Care, Virinchi Hospital, Hyderabad, Telangana, India.
Indian J Crit Care Med. 2021 Mar;25(3):322-326. doi: 10.5005/jp-journals-10071-23765.
To study the correlation between the cycle threshold (CT) of reverse transcription-polymerase chain reaction (RT-PCR) test in confirmed COVID-19 patients and the severity of disease. RT-PCR test is a standard method for the diagnosis of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections. This test is based upon the amplification of the fluorescent signal. The number of cycles that the fluorescent signal undergoes to reach the threshold is called "cycle threshold." It is inversely related to the nucleic acid content of the sample. This is a single-centered, retrospective observational study. We have included a total of 192 patients. SARS-CoV-2 infection was confirmed by the RT-PCR test. Entire data have been collected from the electronic medical records. The primary outcome was 28-day mortality, whereas the secondary outcomes were intensive care unit (ICU) admission, invasive ventilation, acute kidney injury, renal replacement therapy (RRT), shock, and COVID-19 reporting and data system (CO-RADS) score on high-resolution computed tomography of the chest, total length of stay in the hospital, and the number of ICU days and ventilator days. We have calculated the mean CT value for all groups and calculated the -value for statistical significance. For the total length of stay in the hospital and the number of ICU days and ventilator days, we applied the Pearson correlation coefficient. The -value was statistically significant for mortality, ICU admission, and shock groups. The CT values and the length of ICU stay were inversely correlated with the statistically significant -value. Low CT value is associated with increased ICU admission, high mortality, shock, and increased length of ICU stay. Rajyalakshmi B, Samavedam S, Reddy PR, Aluru N. Prognostic Value of "Cycle Threshold" in Confirmed COVID-19 Patients. Indian J Crit Care Med 2021;25(3):322-326.
研究确诊新型冠状病毒肺炎（COVID-19）患者的逆转录聚合酶链反应（RT-PCR）检测的循环阈值（CT）与疾病严重程度之间的相关性。RT-PCR检测是诊断严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染的标准方法。该检测基于荧光信号的扩增。荧光信号达到阈值所经历的循环次数称为“循环阈值”。它与样本的核酸含量呈负相关。这是一项单中心回顾性观察研究。我们共纳入了192例患者。SARS-CoV-2感染通过RT-PCR检测确诊。全部数据均从电子病历中收集。主要结局为28天死亡率，次要结局为重症监护病房（ICU）入住、有创通气、急性肾损伤、肾脏替代治疗（RRT）、休克以及胸部高分辨率计算机断层扫描的COVID-19报告和数据系统（CO-RADS）评分、住院总时长、ICU天数和呼吸机使用天数。我们计算了所有组的平均CT值并计算了具有统计学意义的P值。对于住院总时长、ICU天数和呼吸机使用天数，我们应用了Pearson相关系数。P值在死亡率、ICU入住和休克组中具有统计学意义。CT值与ICU住院时长呈负相关，P值具有统计学意义。低CT值与ICU入住增加、高死亡率、休克以及ICU住院时长增加相关。拉贾亚拉克希米·B、萨马韦丹·S、雷迪·P·R、阿鲁鲁·N。确诊COVID-19患者中“循环阈值”的预后价值。《印度重症监护医学杂志》2021年；25（3）：322 - 326。
