Silveira Melise Chaves, Rocha-de-Souza Cláudio Marcos, de Oliveira Santos Ivson Cassiano, Pontes Leilane da Silva, Oliveira Thamirys Rachel Tavares E, Tavares-Teixeira Camila Bastos, Cossatis Nataly de Almeida, Pereira Natacha Ferreira, da Conceição-Neto Orlando Carlos, da Costa Bianca Santos, Rodrigues Daiana Cristina Silva, Albano Rodolpho Mattos, da Silva Fabrício Alves Barbosa, Marques Elizabeth Andrade, Leão Robson Souza, Carvalho-Assef Ana Paula D'Alincourt
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, Brazil.
Departamento de Bioquímica, Instituto de Biologia Roberto de Alcântara Gome, Universidade do Estado do Rio de Janeiro - UERJ, Rio de Janeiro, Brazil.
Front Med (Lausanne). 2021 Mar 15;8:635206. doi: 10.3389/fmed.2021.635206. eCollection 2021.
Multidrug-resistant microorganisms are a well-known global problem, and gram-negative bacilli are top-ranking. When these pathogens are associated with bloodstream infections (BSI), outcomes become even worse. Here we applied whole-genome sequencing to access information about clonal distribution, resistance mechanism diversity and other molecular aspects of gram-negative bacilli (GNB) isolated from bloodstream infections in Brazil. It was possible to highlight international high-risk clones circulating in the Brazilian territory, such as CC258 for , ST79 for and ST233 for . Important associations can be made such as a negative correlation between CRISPR-Cas and CC258, while the genes , and are highly associated with this clone. Specific relationships between clones and variants were also observed. All ST233 isolates showed the genes and . In addition, some trends could be identified, where a new MDR clone (ST3079), a novel clonal profile circulating in Brazil (ST848), and important resistance associations in the form of and being found together in one ST233 strain, stand out. Such findings may help to develop approaches to deal with BSI and even other nosocomial infections caused by these important GNB.
多重耐药微生物是一个众所周知的全球性问题，革兰氏阴性杆菌位居榜首。当这些病原体与血流感染（BSI）相关时，后果会更糟。在这里，我们应用全基因组测序来获取有关从巴西血流感染中分离出的革兰氏阴性杆菌（GNB）的克隆分布、耐药机制多样性和其他分子方面的信息。有可能突出在巴西境内传播的国际高危克隆，例如 的CC258、 的ST79和 的ST233。可以建立重要的关联，例如CRISPR-Cas与CC258之间呈负相关，而基因 、 和 与该克隆高度相关。还观察到克隆与 变体之间的特定关系。所有ST233分离株均显示出基因 和 。此外，可以确定一些趋势，其中一个新的多重耐药克隆（ST3079）、一种在巴西传播的新型克隆谱（ST848）以及在一个ST233菌株中同时发现的 和 形式的重要耐药关联尤为突出。这些发现可能有助于制定应对BSI甚至其他由这些重要GNB引起的医院感染的方法。
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