School of Food Science and Engineering, South China University of Technology, 381 Wu Shan Road, Guangzhou, 510641, China.
Beijing Scitop Bio-Tech Co. Ltd., Beijing, China.
Eur J Nutr. 2021 Oct;60(7):3757-3769. doi: 10.1007/s00394-021-02543-x. Epub 2021 Apr 1.
Studies have shown that Alzheimer's disease is associated with significant alterations in the gut microbiota. But the effect of probiotics and/or prebiotics on Alzheimer's disease still remains to be explored. The aim of this study was to determine whether Bifidobacterium Lactis Probio-M8 could alleviate Alzheimer's disease pathophysiologies in the APP/PS1 transgenic mouse model.
4-month old APP/PS1 mice were randomly put into two groups and fed with either Probio-M8 or saline water for 45 days. Fecal samples of mice were collected at the beginning and the end of the treatment period to determine the composition of the gut microbiota via 16S ribosomal RNA sequencing technology. The number and size of Aβ plaques in the brain were quantified. In addition, Y maze, novel object recognition and nest building were employed to access cognitive function in the 8-months old APP/PS1 mice at the end of the treatment period.
Our results demonstrated that Probio-M8 reduced Aβ plaque burden in the whole brain and protected against gut microbiota dysbiosis. Furthermore, Probio-M8 could alleviate cognitive impairment in the APP/PS1 mouse.
研究表明，阿尔茨海默病与肠道微生物群的显著改变有关。但益生菌和/或益生元对阿尔茨海默病的影响仍有待探索。本研究旨在确定双歧杆菌 Lactis Probio-M8 是否可以减轻 APP/PS1 转基因小鼠模型中的阿尔茨海默病病理。
将 4 月龄的 APP/PS1 小鼠随机分为两组，分别用 Probio-M8 或生理盐水喂养 45 天。在治疗开始和结束时收集小鼠的粪便样本，通过 16S 核糖体 RNA 测序技术确定肠道微生物群的组成。定量分析大脑中 Aβ 斑块的数量和大小。此外，在治疗结束时，采用 Y 迷宫、新物体识别和筑巢来评估 8 月龄 APP/PS1 小鼠的认知功能。
我们的结果表明，Probio-M8 降低了全脑 Aβ 斑块负担，并防止了肠道微生物群失调。此外，Probio-M8 可以减轻 APP/PS1 小鼠的认知障碍。
