Department of Biological Sciences, Boise State University, Boise, ID, 83725, USA.
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA.
Microb Ecol. 2022 Jan;83(1):167-181. doi: 10.1007/s00248-021-01739-2. Epub 2021 Apr 2.
Host-microbiome dynamics occurring in the yellow fever mosquito (Aedes aegypti) contribute to host life history traits, and particular bacterial taxa are proposed to comprise a "core" microbiota that influences host physiology. Laboratory-based studies are frequently performed to investigate these processes; however, experimental results are often presumed to be generalizable across laboratories, and few efforts have been made to independently reproduce and replicate significant findings. A recent study by Muturi et al. (FEMS Microbiol Ecol 95 (1):213, 2019) demonstrated the food source imbibed by laboratory-reared adult female mosquitoes significantly impacted the host-associated microbiota-a foundational finding in the field of mosquito biology worthy of independent evaluation. Here, we coalesce these data with two additional mosquito-derived 16S rRNA gene sequence data sets using a unifying bioinformatics pipeline to reproduce the characterization of these microbiota, test for a significant food source effect when independent samples were added to the analyses, assess whether similarly fed mosquito microbiomes were comparable across laboratories, and identify conserved bacterial taxa. Our pipeline characterized similar microbiome composition and structure from the data published previously, and a significant food source effect was detected with the addition of independent samples, increasing the robustness of this previously discovered component of mosquito biology. However, distinct microbial communities were identified from similarly fed but independently reared mosquitoes, and surveys across all samples did not identify conserved bacterial taxa. These findings demonstrated that while the main effect of the food source was supported, laboratory-specific conditions may produce inherently differential microbiomes across independent laboratory environments.
在黄热病蚊（埃及伊蚊）中发生的宿主-微生物组动态有助于宿主的生活史特征，并且特定的细菌分类群被提议构成影响宿主生理的“核心”微生物组。经常在实验室中进行基于实验室的研究来研究这些过程；然而，实验结果通常被假定可以在实验室之间推广，并且很少有人努力独立复制和复制重要的发现。Muturi 等人最近的一项研究。（FEMS Microbiol Ecol 95（1）：213，2019）表明，实验室饲养的成年雌性蚊子所摄取的食物来源显著影响了与宿主相关的微生物组-这是蚊子生物学领域值得独立评估的基础发现。在这里，我们使用统一的生物信息学管道将这些数据与另外两个蚊子衍生的 16S rRNA 基因序列数据集合并，以重现这些微生物组的特征，当将独立样本添加到分析中时测试食物来源的显著影响，评估在不同实验室中是否可以比较类似喂养的蚊子微生物组，并鉴定保守的细菌分类群。我们的管道从先前发表的数据中描述了相似的微生物组组成和结构，并且通过添加独立样本检测到了食物来源的显著影响，从而提高了先前发现的蚊子生物学成分的稳健性。然而，从类似喂养但独立饲养的蚊子中鉴定出了不同的微生物群落，并且对所有样本的调查并未鉴定出保守的细菌分类群。这些发现表明，虽然支持了食物来源的主要影响，但实验室特定的条件可能会在独立的实验室环境中产生固有差异的微生物组。
