Thuan Nguyen Huy, Shrestha Anil, Trung Nguyen Thanh, Tatipamula Vinay Bharadwaj, Van Cuong Duong, Canh Nguyen Xuan, Van Giang Nguyen, Kim Tae-Su, Sohng Jae Kyung, Dhakal Dipesh
Institute of Research and Development, Duy Tan University, Da Nang, Vietnam.
Combinatorial Biosynthesis National Research Laboratory, Ewha Womans University, Seoul, Republic of Korea.
Biotechnol Appl Biochem. 2022 Apr;69(2):848-861. doi: 10.1002/bab.2156. Epub 2021 Apr 14.
Taxifolin (dihydroquercetin) and its derivatives are medicinally important flavanonols with a wide distribution in plants. These compounds have been isolated from various plants, such as milk thistle, onions, french maritime, and tamarind. In general, they are commercially generated in semisynthetic forms. Taxifolin and related compounds are biosynthesized via the phenylpropanoid pathway, and most of the biosynthetic steps have been functionally characterized. The knowledge gained through the detailed investigation of their biosynthesis has provided the foundation for the reconstruction of biosynthetic pathways. Plant- and microbial-based platforms are utilized for the expression of such pathways for generating taxifolin-related compounds, either by whole-cell biotransformation or through reconfiguration of the genetic circuits. In this review, we summarize recent advances in the biotechnological production of taxifolin and its derivatives.
紫杉叶素（二氢槲皮素）及其衍生物是具有重要药用价值的黄烷醇类化合物，在植物中广泛分布。这些化合物已从多种植物中分离出来，如乳蓟、洋葱、法国滨海植物和罗望子。一般来说，它们以半合成形式进行商业生产。紫杉叶素及相关化合物通过苯丙烷途径生物合成，并且大多数生物合成步骤已得到功能表征。通过对其生物合成的详细研究获得的知识为生物合成途径的重建提供了基础。基于植物和微生物的平台被用于表达此类途径以生成紫杉叶素相关化合物，无论是通过全细胞生物转化还是通过基因回路的重新配置。在本综述中，我们总结了紫杉叶素及其衍生物生物技术生产的最新进展。
