Al-Jameel Suhailah S, Almessiere Munirah A, Khan Firdos A, Taskhandi Nedaa, Slimani Yassine, Al-Saleh Najat S, Manikandan Ayyar, Al-Suhaimi Ebtesam A, Baykal Abdulhadi
Department of Chemistry, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
Department of Biophysics, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
Nanomaterials (Basel). 2021 Mar 11;11(3):700. doi: 10.3390/nano11030700.
There is enormous interest in combining two or more nanoparticles for various biomedical applications, especially in anti-cancer agent delivery. In this study, the microsphere nanoparticles were prepared (MSNPs) and their impact on cancer cells was examined. The MSNPs were prepared by using the hydrothermal method where strontium (Sr), barium (Ba), dysprosium (Dy), samarium (Sm), and iron oxide (FeO) were combined, and dysprosium (Dy) and samarium (Sm) was substituted with strontium (Sr) and barium (Ba), preparing SrBaDySmFeO (0.00 ≤ ≤ 1.0) MSNPs. The microspheres were characterized by X-ray powder diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques. The diffraction pattern of nanohexaferrites (NHFs) reflected the signature peaks of the hexagonal structure. The XRD revealed a pure hexagonal structure without any undesired phase, which indicated the homogeneity of the products. The crystal size of the nanoparticles were in the range of 22 to 36 nm by Scherrer's equation. The SEM of MSNPs showed a semi-spherical shape with a high degree of aggregation. TEM and HR-TEM images of MSNPs verified the spherical shape morphology and structure that approved an M-type hexaferrite formation. The anti-cancer activity was examined on HCT-116 (human colorectal carcinoma) and HeLa (cervical cancer cells) using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and post-48 h treatment of MSNPs caused a dose-dependent inhibition of HCT-116 and HeLa cell proliferation and growth. Conversely, no significant cytotoxic effect was observed on HEK-293 cells. The treatments of MSNPs also induced cancer cells DNA disintegration, as revealed by 4',6-diamidino-2-phenylindole (DAPI) staining. Finally, these findings suggest that synthesized MSNPs possess potential inhibitory actions on cancerous cells without harming normal cells.
将两种或更多种纳米颗粒结合用于各种生物医学应用，尤其是在抗癌药物递送方面，引起了极大的关注。在本研究中，制备了微球纳米颗粒（MSNPs）并检测了它们对癌细胞的影响。MSNPs采用水热法制备，将锶（Sr）、钡（Ba）、镝（Dy）、钐（Sm）和氧化铁（FeO）结合在一起，并用锶（Sr）和钡（Ba）替代镝（Dy）和钐（Sm），制备了SrBaDySmFeO（0.00 ≤ ≤ 1.0）MSNPs。通过X射线粉末衍射（XRD）、高分辨率透射电子显微镜（HR-TEM）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和能量色散X射线光谱（EDX）技术对微球进行了表征。纳米六铁氧体（NHFs）的衍射图谱反映了六方结构的特征峰。XRD显示为纯六方结构，无任何不需要的相，这表明产物的均匀性。根据谢乐方程，纳米颗粒的晶体尺寸在22至36nm范围内。MSNPs的SEM显示为高度聚集的半球形。MSNPs的TEM和HR-TEM图像证实了球形形态和结构，证实形成了M型六铁氧体。使用MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法检测了对HCT-116（人结肠癌细胞）和HeLa（宫颈癌细胞）的抗癌活性，MSNPs处理48小时后对HCT-116和HeLa细胞的增殖和生长产生了剂量依赖性抑制。相反，在HEK-293细胞上未观察到明显的细胞毒性作用。4',6-二脒基-2-苯基吲哚（DAPI）染色显示，MSNPs处理还诱导癌细胞DNA解体。最后，这些发现表明合成的MSNPs对癌细胞具有潜在的抑制作用，而不会损害正常细胞。
