Al Baroot Abbad, Elsayed Khaled A, Khan Firdos Alam, Haladu Shamsuddeen A, Ercan Filiz, Çevik Emre, Drmosh Q A, Almessiere M A
Department of Basic Engineering Sciences, College of Engineering, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
Department of Stem Cell Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
Micromachines (Basel). 2023 Jul 20;14(7):1455. doi: 10.3390/mi14071455.
Gold nanoparticles (AuNPs) and carbon nanotubes (CNTs) are increasingly being investigated for cancer management due to their physicochemical properties, low toxicity, and biocompatibility. This study used an eco-friendly technique (laser synthesis) to fabricate AuNP and Au/CNT nanocomposites. AuNPs, Au/CNTs, and CNTs were tested as potential cancer nanotherapeutics on colorectal carcinoma cells (HCT-116) and cervical cancer cells (HeLa) using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. In addition, the non-cancer embryonic kidney cells HEK-293 were taken as a control in the study. The cell viability assay demonstrated a significant reduction in cancer cell population post 48 h treatments of AuNPs, and Au/CNTs. The average cell viabilities of AuNPs, Au/CNTs, and CNTs for HCT-116 cells were 50.62%, 65.88%, 93.55%, and for HeLa cells, the cell viabilities were 50.88%, 66.51%, 91.73%. The cell viabilities for HEK-293 were 50.44%, 65.80%, 93.20%. Both AuNPs and Au/CNTs showed higher cell toxicity and cell death compared with CNT nanomaterials. The treatment of AuNPs and Au/CNTs showed strong inhibitory action on HCT-116 and HeLa cells. However, the treatment of CNTs did not significantly decrease HCT-116 and HeLa cells, and there was only a minor decrease. The treatment of AuNPs, and Au/CNTs, on normal HEK-293 cells also showed a significant decrease in cell viability, but the treatment of CNTs did not produce a significant decrease in the HEK-293 cells. This study shows that a simplified synthesis technique like laser synthesis for the preparation of high-purity nanomaterials has good efficacy for possible future cancer therapy with minimal toxicity.
由于金纳米颗粒（AuNPs）和碳纳米管（CNTs）的物理化学性质、低毒性和生物相容性，它们在癌症治疗方面越来越受到研究。本研究采用一种环保技术（激光合成）来制备AuNP和Au/CNT纳米复合材料。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四氮唑溴盐（MTT）测定法，将AuNPs、Au/CNTs和CNTs作为潜在的癌症纳米治疗剂对结肠癌细胞（HCT-116）和宫颈癌细胞（HeLa）进行测试。此外，本研究将非癌胚胎肾细胞HEK-293作为对照。细胞活力测定表明，在对AuNPs和Au/CNTs进行48小时处理后，癌细胞数量显著减少。HCT-116细胞中AuNPs、Au/CNTs和CNTs的平均细胞活力分别为50.62%、65.88%、93.55%，HeLa细胞的细胞活力分别为50.88%、66.51%、91.73%。HEK-293细胞的细胞活力分别为50.44%、65.80%、93.20%。与CNT纳米材料相比，AuNPs和Au/CNTs均表现出更高的细胞毒性和细胞死亡。AuNPs和Au/CNTs的处理对HCT-116和HeLa细胞显示出强烈的抑制作用。然而，CNTs的处理并未显著降低HCT-116和HeLa细胞的数量，只是略有减少。AuNPs和Au/CNTs对正常HEK-293细胞的处理也显示出细胞活力显著降低，但CNTs的处理并未使HEK-293细胞产生显著降低。本研究表明，像激光合成这样的简化合成技术用于制备高纯度纳米材料，对于未来可能的癌症治疗具有良好的疗效且毒性最小。
