Favresse Julien, Eucher Christine, Elsen Marc, Gillot Constant, Van Eeckhoudt Sandrine, Dogné Jean-Michel, Douxfils Jonathan
Department of Laboratory Medicine, Clinique St-Luc Bouge, 5004 Namur, Belgium.
Department of Pharmacy, Namur Research Institute for Life Sciences, University of Namur, 5000 Namur, Belgium.
Microorganisms. 2021 Mar 8;9(3):556. doi: 10.3390/microorganisms9030556.
Several studies have described the long-term kinetics of anti-SARS-CoV-2 antibodies but long-term follow-up data, i.e., >6 months, are still sparse. Additionally, the literature is inconsistent regarding the waning effect of the serological response. The aim of this study was to explore the temporal dynamic changes of the immune response after SARS-CoV-2 infection in hospitalized and non-hospitalized symptomatic patients over a period of 10 months. Six different analytical kits for SARS-CoV-2 antibody detection were used. Positivity rates, inter-assay agreement and kinetic models were determined. A high inter-individual and an inter-methodology variability was observed. Assays targeting total antibodies presented higher positivity rates and reached the highest positivity rates sooner compared with assays directed against IgG. The inter-assay agreement was also higher between these assays. The stratification by disease severity showed a much-elevated serological response in hospitalized versus non-hospitalized patients in all assays. In this 10-month follow-up study, serological assays showed a clinically significant difference to detect past SARS-CoV-2 infection with total antibody assays presenting the highest positivity rates. The waning effect reported in several studies should be interpreted with caution because it could depend on the assay considered.
多项研究描述了抗SARS-CoV-2抗体的长期动力学，但长期随访数据，即超过6个月的数据仍然稀少。此外，关于血清学反应减弱效应的文献并不一致。本研究的目的是探讨住院和非住院有症状患者在感染SARS-CoV-2后10个月内免疫反应的时间动态变化。使用了六种不同的SARS-CoV-2抗体检测分析试剂盒。测定了阳性率、检测间一致性和动力学模型。观察到个体间和方法间存在高度变异性。与针对IgG的检测相比，针对总抗体的检测呈现出更高的阳性率，且更早达到最高阳性率。这些检测之间的检测间一致性也更高。按疾病严重程度分层显示，在所有检测中，住院患者与非住院患者相比血清学反应明显升高。在这项为期10个月的随访研究中，血清学检测显示，总抗体检测在检测既往SARS-CoV-2感染方面呈现出最高的阳性率，具有临床显著差异。几项研究中报道的减弱效应应谨慎解读，因为它可能取决于所考虑的检测方法。
