Akramov Davlat Kh, Mamadalieva Nilufar Z, Porzel Andrea, Hussain Hidayat, Dube Mthandazo, Akhmedov Akbar, Altyar Ahmed E, Ashour Mohamed L, Wessjohann Ludger A
Institute of the Chemistry of Plant Substances, Uzbekistan Academy of Sciences, M. Ulugbek Str 77, Tashkent 100170, Uzbekistan.
Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
Molecules. 2021 Mar 21;26(6):1755. doi: 10.3390/molecules26061755.
Phytochemical investigation of the methanolic extract obtained from the aerial parts of (Lamiaceae) afforded the new compound 1-methoxy-3---glucopyranosyl-α-l-oliose () together with five known glycosides, namely sitosterol-3---glucoside (), stigmasterol-3---glucoside (), pinitol (), 6-hydroxyl-7--loganin (), and chlorotuberoside (). The structures of these compounds were elucidated by extensive spectroscopic analyses, especially HR-MS, 1D and 2D NMR spectroscopy. The in vitro cytotoxic activity of the methanolic extract and the isolated compounds was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and crystal violet (CV) staining assays. In addition, the antifungal activities of the components were evaluated against and The anthelmintic potential was determined against nematodes. Neither the extract nor the isolated compounds showed promising activity in all the bioassays.
对唇形科植物地上部分的甲醇提取物进行植物化学研究，得到了新化合物1-甲氧基-3-O-β-D-吡喃葡萄糖基-α-L-橄榄糖（）以及五种已知糖苷，即β-谷甾醇-3-O-β-D-葡萄糖苷（）、豆甾醇-3-O-β-D-葡萄糖苷（）、松醇（）、6-羟基-7-O-β-D-龙胆二糖苷（）和氯薯蓣皂苷（）。通过广泛的光谱分析，尤其是高分辨质谱、一维和二维核磁共振光谱对这些化合物的结构进行了阐明。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑（MTT）和结晶紫（CV）染色试验评估了甲醇提取物和分离出的化合物的体外细胞毒性活性。此外，还评估了这些成分对白色念珠菌和酿酒酵母的抗真菌活性。针对秀丽隐杆线虫测定了驱虫潜力。提取物和分离出的化合物在所有生物测定中均未显示出有前景的活性。
