Programa de Pós-graduação em Química, Instituto de Química, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica, RJ, Brazil.
Programa de Pós-graduação em Química, Instituto de Química, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica, RJ, Brazil.
Phytochemistry. 2019 Jun;162:250-259. doi: 10.1016/j.phytochem.2019.03.010. Epub 2019 Apr 6.
Seven undescribed dammarane-type triterpenoids, together with ten known compounds, were isolated from the stems of Ziziphus glaziovii Warm (= Sarcomphalus glaziovii (Warm.) Hauenschild). The structures were fully assigned by means of uni- and bidimensional NMR and HR-ESI-MS experiments. Extract, fractions and also isolated compounds were evaluated for their antibacterial (against Bacillus subtilis and Aliivibrio fischeri), cytotoxic (against PC-3 and HT-29 human cancer cell lines), anthelmintic (against Caenorhabditis elegans) and antifungal (against Septoria triciti, Botrytis cinerea and Phytopthoria infestans) activities. The methanolic crude extract exhibited substantial antibacterial and cytotoxic activity. The known triterpenes epigouanic acid and alphitolic acid were the most active compounds against B. subtilis, with IC of 12 and 22 μM, respectively. The isolated compounds presented up to a concentration of 10 μM none or only weak effects in the cytotoxicity assays. No anthelminthic and antifungal activities were observed.
从酸枣（Ziziphus glaziovii Warm.） (= Sarcomphalus glaziovii (Warm.) Hauenschild）的茎中分离得到了七种未描述的达玛烷型三萜类化合物，以及十种已知化合物。通过一维和二维 NMR 和 HR-ESI-MS 实验，对结构进行了全面解析。提取物、馏分和分离得到的化合物都进行了抗菌（对枯草芽孢杆菌和发光弧菌）、细胞毒性（对 PC-3 和 HT-29 人癌细胞系）、驱虫（对秀丽隐杆线虫）和抗真菌（对三叶草叶斑病菌、灰葡萄孢菌和马铃薯晚疫病菌）活性评价。甲醇粗提取物表现出较强的抗菌和细胞毒性活性。已知的三萜类化合物表高吉酸和阿尔法体酸对枯草芽孢杆菌的活性最强，IC 分别为 12 和 22µM。分离得到的化合物在细胞毒性测定中，最高浓度为 10µM 时，没有或仅有微弱的活性。未观察到驱虫和抗真菌活性。
