Key Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Genes (Basel). 2021 Mar 21;12(3):449. doi: 10.3390/genes12030449.
Green chrysanthemums are difficult to breed but have high commercial value. The molecular basis for the green petal color in chrysanthemum is not fully understood. This was investigated in the present study by RNA sequencing analysis of white and green ray florets collected at three stages of flower development from the F progeny of the cross between "Lüdingdang" with green-petaled flowers and with white-petaled flowers. The chlorophyll content was higher and chloroplast degradation was slower in green pools than in white pools at each developmental stage. Transcriptome analysis revealed that genes that were differentially expressed between the two pools were enriched in pathways related to chlorophyll metabolism and photosynthesis. We identified the transcription factor genes , , , and as regulators of the green flower color in chrysanthemum by differential expression analysis and weighted gene co-expression network analysis. These findings can guide future efforts to improve the color palette of chrysanthemum flowers through genetic engineering.
绿菊难以繁殖，但具有很高的商业价值。菊花绿色花瓣的分子基础尚不完全清楚。本研究通过对“绿定档”与白花瓣花杂交的 F 代后代的白色和绿色射线花瓣在花发育的三个阶段采集的 RNA 测序分析，对此进行了研究。在每个发育阶段，绿色花池中叶绿素含量较高，叶绿体降解速度较慢。转录组分析表明，两个池中差异表达的基因在与叶绿素代谢和光合作用相关的途径中富集。通过差异表达分析和加权基因共表达网络分析，我们确定转录因子基因 、 、 、 是菊花绿色花色的调控因子。这些发现可以指导未来通过基因工程改善菊花花色的努力。
