Jakubowski Witold, Atraszkiewicz Radomir, Nowak Dorota, Batory Damian, Szymański Witold, Sobczyk-Guzenda Anna, Kaczmarek Łukasz, Kula Piotr, Cłapa Marian, Warga Tomasz, Czerniak-Reczulska Małgorzata
Division of Biophysics, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
Division of Surface Engineering and Heat Treatment, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
Nanomaterials (Basel). 2021 Mar 17;11(3):756. doi: 10.3390/nano11030756.
The presented work shows the results of the functionalization of the graphene surface obtained by the growth on the liquid bimetallic matrices method. We used glutathione (GSH) as a peptide model, which allowed us to optimize the procedure to obtain high process efficiency. To establish the amount of GSH attached to the graphene surface, the Folina-Ciocalteu method was used, which allows the assessment of the concentration of colored reaction products with peptide bonds without the disadvantages of most methods based on direct colored reaction of peptide bonds. Samples surface morphology, quality of graphene and chemical structure in the subsequent stages of surface modification were tested-for this purpose Raman spectroscopy, scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR) were used.
本文展示了通过在液态双金属基质上生长法获得的石墨烯表面功能化的结果。我们使用谷胱甘肽（GSH）作为肽模型，这使我们能够优化程序以获得高工艺效率。为了确定附着在石墨烯表面的GSH的量，使用了Folina-Ciocalteu方法，该方法可以评估具有肽键的有色反应产物的浓度，而没有大多数基于肽键直接显色反应的方法的缺点。在表面改性的后续阶段测试了样品的表面形态、石墨烯质量和化学结构，为此使用了拉曼光谱、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）。
